| dc.description.abstract | The present study was aimed to determinate the seroprevalence of Peste des petits
ruminants (PPR) of sheep in Dinajpur district. The study was conducted at the Department
of Pathology and Microbiology lab, Faculty of Veterinary and Animal Science, Hajee
Mohammed Danesh of Science and Technology University (HSTU), Dinajpur for a period
of 6 months from January to June, 2018. Blood samples were collected from sheep by
jugular venipuncture method from sheep by using 5 ml syringe in a test tube without any
anticoagulant without previous history of peste des petits ruminants PPR vaccination. The
total blood samples were 66 and the samples were collected according to area, aged group,
sex breed, lactation stage and parity. For serum collection the samples were kept for an
hour at room temperature in a slightly inclined position to facilitate clotting, after clotting
serum was separated and stored at -20 0C until use. The result showed that the overall
prevalence in sheep was 24.24%. The present study revealed that the prevalence of PPR
was not significantly (P= 0.869) differed based on area and the result showed that the
highest seropositive (27.27%) was observed in Chirirbandar upazila whereas the lowest
seropositivity (15.38 %) was detected from Sadar, Dinajpur. However the other locations
were intermediate. In respect to sex, there was no significant variation (P= 0.935), and it
was found that the seropositive percentage was not so higher in male sheep (25%) than
female sheep (24%). According to the aged group, there was no significant effect among
aged group (P= 0.561) and the result exhibited that the highest PPRV seropositive in sheep
(38.46%) was in the first group (<1 year) while lowest seropositive (16.66%) were found
from the third group (>3-6 years). According to lactation stage prevalence of PPR was
higher in lactating stage or post partum stage (33.33%) than lamb (30%) and non lactating
stage (13.63%) respectively. The prevalence of PPRV was not significantly (P=0.308)
differed according to lactation stage. Based on parity, the prevalence of PPR was
insignificantly higher in third parity (50.00%) where the other parity were no parity
(30.00%), 1st parity (23.08%), 2nd parity (9.09%), 4th parity (40.00%) and there was no
significant (P = 0.302) effect on prevalence of PPR in sheep based on parity. Competitive
enzyme-linked immunosorbent assay (cELISA) was detected and measured antibody level
against the PPR virus in the sera and the study was suggested that the PPRV was widely
distributed in the study area. Therefore, it is recommended to control PPR virus such as by
using regular and routine homogeneous vaccination should intensify in the study area. | en_US |
