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    • Dept. of Pathology & Parasitology
    • Masters Thesis
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    •   HSTUL IR
    • Faculty of Veterinary and Animal Science
    • Dept. of Pathology & Parasitology
    • Masters Thesis
    • View Item
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    SEROPREVALENCE OF PESTE DES PETITS RUMINANTS (PPR) IN SHEEP AT DINAJPUR DISTRICT

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    AFSANA SIDDIKA REGISTRATION NO.: 1605471 SEMESTER: JANUARY-JUNE, 2018 SESSION: 2016-2017 (2.924Mb)
    Date
    2018-06
    Author
    SIDDIKA, AFSANA
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    URI
    http://localhost:8080/xmlui/handle/123456789/1104
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    • Masters Thesis
    Abstract
    The present study was aimed to determinate the seroprevalence of Peste des petits ruminants (PPR) of sheep in Dinajpur district. The study was conducted at the Department of Pathology and Microbiology lab, Faculty of Veterinary and Animal Science, Hajee Mohammed Danesh of Science and Technology University (HSTU), Dinajpur for a period of 6 months from January to June, 2018. Blood samples were collected from sheep by jugular venipuncture method from sheep by using 5 ml syringe in a test tube without any anticoagulant without previous history of peste des petits ruminants PPR vaccination. The total blood samples were 66 and the samples were collected according to area, aged group, sex breed, lactation stage and parity. For serum collection the samples were kept for an hour at room temperature in a slightly inclined position to facilitate clotting, after clotting serum was separated and stored at -20 0C until use. The result showed that the overall prevalence in sheep was 24.24%. The present study revealed that the prevalence of PPR was not significantly (P= 0.869) differed based on area and the result showed that the highest seropositive (27.27%) was observed in Chirirbandar upazila whereas the lowest seropositivity (15.38 %) was detected from Sadar, Dinajpur. However the other locations were intermediate. In respect to sex, there was no significant variation (P= 0.935), and it was found that the seropositive percentage was not so higher in male sheep (25%) than female sheep (24%). According to the aged group, there was no significant effect among aged group (P= 0.561) and the result exhibited that the highest PPRV seropositive in sheep (38.46%) was in the first group (<1 year) while lowest seropositive (16.66%) were found from the third group (>3-6 years). According to lactation stage prevalence of PPR was higher in lactating stage or post partum stage (33.33%) than lamb (30%) and non lactating stage (13.63%) respectively. The prevalence of PPRV was not significantly (P=0.308) differed according to lactation stage. Based on parity, the prevalence of PPR was insignificantly higher in third parity (50.00%) where the other parity were no parity (30.00%), 1st parity (23.08%), 2nd parity (9.09%), 4th parity (40.00%) and there was no significant (P = 0.302) effect on prevalence of PPR in sheep based on parity. Competitive enzyme-linked immunosorbent assay (cELISA) was detected and measured antibody level against the PPR virus in the sera and the study was suggested that the PPRV was widely distributed in the study area. Therefore, it is recommended to control PPR virus such as by using regular and routine homogeneous vaccination should intensify in the study area.

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