EXTRACTION AND CHARACTERIZATION OF PROTEIN FROM FRUIT BY PRODUCTS OF JACKFRUIT, MANGO AND LITCHI SEEDS

Abstract

Extraction of protein from fruit by products and characterization their functional properties were analyzed in this study. Protein extracted by using distilled water, sodium hydroxide and hydrochloric acid. All protein isolated from fruit by product such mango, jackfruit and litchi seed Prepared via NaOH extraction which seed protein content was resulted 28.23%, 43.12% and 36.96%, respectively. The highest protein content was obtained from jackfruit seed protein isolated. Instead the moisture content was investigated and obtained the protein extracted from mango seed, jackfruit and litchi seed were resulted in 3.68%, 15.93%, 16.60%, respectively. Where ash content of three different fruits by-product of MSP, JSP and LSP protein isolate shown as 1.36%, 1.23% and 2.07%, respectively. Furthermore, litchi seed protein isolate contained little bit high amount of ash then the other seed. In this study shown high variation of fat content among different three by-products of mango, jackfruit and litchi seed protein isolate, resulted 14%, 2% and 1%, respectively. Solubility of a protein is one of the critical functional attributes required for its use as a food ingredient. The resulted the protein solubility of different protein seeds extraction of (MSP, JSP, LSP), at different pH levels between 2 and 12. The minimum solubility of mango, jackfruit and litchi seeds protein were found 10.91%, 7.14% and 18.68%, respectively at pH 4, while the solubility were showed in similar results at pH 2. The solubility of three different fruit by-product seeds isolated protein increased from pH 6 to pH 12, and the maximum solubility of 27.27%, 21.42% and 20.75%, respectively was obtained at pH 12. The water absorption capacity of protein seed extraction were 1.48, 2.99 and 2.266 ml H2O/g while fat absorption capacity was resulted 1.393 ml oil/g, 1.488 ml oil/g and 1.528 ml oil/g respectively. The overall results showed that by-product protein extraction might be used protein supplement.