Dept. of Medicine, Surgery and Obstetrics

INVESTIGATION OF DISEASES OF COMMERCIAL CHICKEN IN RANGPUR DISTRICT OF BANGLADESH

Fri, 01 Dec 2023 00:00:00 GMT

INVESTIGATION OF DISEASES OF COMMERCIAL CHICKEN IN RANGPUR DISTRICT OF BANGLADESH ANIK, S. M. TARIF-UZ-ZAMAN This study was designed to investigate the diseases of commercial chicken at Mithapukur upazila in Rangpur district of Bangladesh. The research was conducted for a period of six months from November 2022 to April 2023. A total of 870 clinically ill and dead chicken were taken from the study, of which 308 were broiler, 270 were sonali and 292 were layer chickens and the diagnosis was confirmed by postmortem examination of dead chicken. The overall prevalence of viral disease in broiler, sonali and layer chicken were 37.7%, 30.0% and 26.0% and bacterial disease were 20.1%, 15.6% and 42.8% and fungal, protozoan and other diseases were 42.2%, 54.4% and 31.2% respectively. Inclusion body hepatitis 57 (18.5%) in broiler, coccidiosis 88 (32.6%) in sonali chicken and colibacillosis 40 (13.7%) in layer chicken were predominant disease. Broiler chicken of 14-27 days old age group were more affected (63.2%) with inclusion body hepatitis; sonali chicken of 14 40 days’ age were more (60.2%) affected with coccidiosis and layer chicken of more than 101 days age group showed highest prevalence (62.5%) of colibacillosis than other age group. Predominant diseases were more in flock size between 1001-1500, earthen floor type rearing system and in the farms reared by primary educated people. Treatment efficacy against inclusion body hepatitis in broiler, coccidiosis in sonali and colibacillosis in layer was best achieved by treating broiler with a combination of a) Glucose + Sodium-bicarbonate b) Liver tonic (Heparen/Neomeriol) c) Kidney tonic (Nephrocare/Lumix vet) d) Vitamin-C (Rena C/Vita C) (Ascorbic Acid-98%) e) Immunomodulator (Xillian/AIB Vet) and the sonali chicken with a) Diclazuril (dmeria) (2.0%) b) Vitamin-k (Rena K) c) Vitamin-C (Vita C) (Ascorbic Acid-98%) d) Kidney Tonic (Lumix vet) and layer chicken with a) Florfenicol b) Neomycin Sulphate (Neo vet 70) c) Kidney Tonic (Nephrocare) d) Toxin Binder (AquaTox) e) Vitamin E-Selenium (Sel E). It is concluded that different viral, bacterial and protozoan disease are circulating in broiler, sonali and layer chicken and still they are well managed by different commercial drugs in commercial chickens at farm levels. INVESTIGATION OF DISEASES OF COMMERCIAL CHICKEN IN RANGPUR DISTRICT OF BANGLADESH; A THESIS BY S. M. TARIF-UZ-ZAMAN ANIK, REGISTRATION NO.: 1504064, SEMESTER: JULY-DECEMBER, 2023 SESSION: 2021-2022; MASTER OF SCIENCE IN MEDICINE DEPARTMENT OF MEDICINE, SURGERY AND OBSTETRICS, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; DECEMBER, 2023.

IDENTIFICATION, MOLECULAR CONFIRMATION AND ANTIBIOTIC SENSITIVITY OF BACTERIA ISOLATED FROM REPEAT BREEDER COWS IN RANGPUR DIVISION

Fri, 01 Dec 2023 00:00:00 GMT

IDENTIFICATION, MOLECULAR CONFIRMATION AND ANTIBIOTIC SENSITIVITY OF BACTERIA ISOLATED FROM REPEAT BREEDER COWS IN RANGPUR DIVISION AFROJ, MST. MOUSUMI Recently repeat breeding syndrome becomes a significant challenge for dairy producer due to large economic loss in Bangladesh. The present study was conducted from January to June 2023 to observe the prevalence of repeat breeding syndrome, to isolate and identify the bacteria with their molecular confirmation and antibiotic sensitivity causing repeat breeding syndrome in cows in the northern region of Bangladesh. By using a questionnaire and history-taking techniques (more than 10 years old, one calf in life, three subsequent Artificial Insemination (AI) failures to conceive, having no perceptible anomalies of reproductive tract) to interview dairy owners, repeat breeding syndrome was identified. Bacteria were isolated and identified from the cervical mucus of affected cows. For confirmation of the detected bacteria, PCR was used. The agar disc diffusion method was utilized to investigate the antibiotic sensitivity of the detected isolates against widely used antibiotics in vitro. The prevalence of repeat breeding syndrome was 41.33%. The prevalence of E. coli was 40%, Styphylococcus aureus 40% and Klebsiella spp. 10%. Molecular detection of E. coli and Staphylococcus aureus was confirmed by using Eco 223 (F), Eco 455 (R), Sau 234(F) and Sau 1501(R) primers. The target genes were 16S and 23S rRNA and the size of the product amplified at 232 bp and 1267 bp respectively. According to the antibiogram profile, E. coli was resistant to ciprofloxacin, penicillin, tetracycline, amoxicillin, and erythromycin, but sensitive to ceftriaxone and gentamicin. While Staphylococcus aureus was resistant to ampicillin and erythromycin, it was sensitive to ciprofloxacin, ceftriaxone, and levofloxacin. It was also intermediately resistant to vancomycin and amoxicillin. Klebsiella spp. showed resistance to ceftriaxone, penicillin, and amoxicillin, while remaining sensitive to ciprofloxacin, tetracycline, and intermediate resistance to erythromycin and gentamicin. It should be concluded that E. coli, Styphylococcus aureus and Klebsiella spp. are the most common bacteria causing repeat breeding syndrome in cows. Treatment for repeat breeding syndrome should be done after performing antibiotic sensitivity test. IDENTIFICATION, MOLECULAR CONFIRMATION AND ANTIBIOTIC SENSITIVITY OF BACTERIA ISOLATED FROM REPEAT BREEDER COWS IN RANGPUR DIVISION; A THESIS BY MST. MOUSUMI AFROJ, REGISTRATION NO.: 1504025, SEMESTER: JULY-DECEMBER, 2023 SESSION: 2022-2023; MASTER OF SCIENCE (MS) IN THERIOGENOLOGY, DEPARTMENT OF MEDICINE, SURGERY AND OBSTETRICS , HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; DECEMBER 2023.

PREGNANCY DIAGNOSIS IN GOAT BY USING VAGINAL CYTOLOGY AND TRANS-ABDOMINAL ULTRASONOGRAPHY

Fri, 01 Jun 2018 00:00:00 GMT

PREGNANCY DIAGNOSIS IN GOAT BY USING VAGINAL CYTOLOGY AND TRANS-ABDOMINAL ULTRASONOGRAPHY The present study was conducted in a goat farm for pregnancy detection by using vaginal cytology and B-mode real time ultrasound using 5 MHz probe by transabdominal method. Seventeen pregnant does were used for this study. The objective of the study was to determine the earliest days of pregnancy and describe the chronological characteristics of pregnancy from days 22 to days 40 for vaginal cytology and days 25 to days 60 for ultrasonography of gestation. The present study revealed that the pregnancy was detected at 22-25 days after mating by detection of higher average percentages of intermediate cells and few percentage of superficial, keratinized and neutrophil cells. Parabasal cells were absent. The average percentage of intermediate, superficial, keratinized and neutrophil cells during early stage of pregnancy were 81.12%, 9.41%, 7.10% and 2.61% respectively on 22-40th days. In case of real time B-mode ultrasonography, pregnancy was detected at 25 days after mating by detection of gestational sac. The gestational sac was observed only in three does out of seventeen (17.6 %) at 25-30 days whereas the placentomes & heart beat of the foetus were first detected at 31-35 days in six does (35.3%). The foetal leg buds were first visualized at 36-40 days in four does (23.5%) whereas the foetal vertebral column was first observed at 36-40 days of gestation in only three does (17.6%). It could be concluded that vaginal cytology may be used for detection of pregnancy in doe. The real time transabdominal ultrasonography pregnancy can be easily utilized for early pregnancy diagnosis in does at field level. Further work involving large number of animal throughout the pregnant period should be needed. A THESIS BY MD. AZIZ ALI REGISTRATION NO.: 1605500 SEMESTER: JANUARY-JUNE, 2018 SESSION: 2016-2017 Submitted to the Department of Medicine, Surgery and Obstetrics Faculty of Veterinary and Animal Science Hajee Mohammad Danesh Science and Technology University, Dinajpur5200 In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN THERIOGENOLOGY

SCROTAL CIRCUMFERENCE AND ITS RELATIONSHIP WITH TESTICULAR GROWTH, AGE AND BODY WEIGHT IN INDIGENOUS BULLS

Fri, 01 Jun 2018 00:00:00 GMT

SCROTAL CIRCUMFERENCE AND ITS RELATIONSHIP WITH TESTICULAR GROWTH, AGE AND BODY WEIGHT IN INDIGENOUS BULLS ALI, MD. ROMJAN The relationship among the scrotal circumference and testicular parameters with body weight and age were investigated in indigenous bulls. Data from thirty six indigenous bulls were recorded and classified into four age groups namely, Group I (15-18 months, n=7 animals), Group II (19-22 months, n=8 animals), Group III (23-26 months n=12 animals) and Group IV (27 months and above, n=9 animals). Age of the bulls were determined using their dental formula. Live weight body weight of each bull was calculated according to Shaeffer’s formula. Scrotal circumference were measured with a cloth tape and testicular parameters and were measured with a caliper. Body weight, scrotal circumference and testicular parameters were varied in different age groups even in left and right testes of the same animal. The results of different testicular parameters, scrotal circumference, and body weight at different age of Indigenous bulls were significantly (<0.05) different among the age groups. These parameters were increased by the advancement of age. The body weight was lowest in case of age group I (98.14±11.87 kg) and was highest in age group IV (214.22±33.27 kg). The scrotal circumference was lowest in case of age group I (17.14±.63 cm) and was highest in age group IV (24.33±1.31 cm). The average estimated testicular weight was lowest in age group I (86.38±12.18 gm) and was highest in age group IV (214.03±22.93 gm). Various measurements of the testes were highly correlated with each other (< 0.01). Body weight was more highly correlated with the various testicular measurements than age. Moreover age, testis weight, and body weight of Indigenous bulls were highly correlated with scrotal circumference. Scrotal circumference was highly correlated with other testicular measurements as well as testicular volume. Correlation coefficient R2 between scrotal circumference and age in indigenous bulls was 0.599 and between scrotal circumference and body weight was 0.785 which indicates a strong positive relationship among the parameters. Finally from the present study it could be concluded that scrotal circumference is a useful indicator and is an important selection criterion to determine the testicular development and breeding soundness in young bulls. A THESIS BY MD. ROMJAN ALI REGISTRATION NO.: 1605501 SEMESTER: JULY-DECEMBER, 2017 SESSION: 2016-2017 Submitted to the Department of Medicine, Surgery and Obstetrics Faculty of Post Graduate Studies Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200 In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN THERIOGENOLOGY