http://103.7.193.12:8080/xmlui/handle/123456789/5 2026-07-26T08:18:32Z http://103.7.193.12:8080/xmlui/handle/123456789/2170 GENETIC IDENTIFICATION AND INDUCED BREEDING OF THREATENED INDIGENOUS SPECIES, Channa striata (Bloch, 1793) FOR SEED PRODUCTION PERVIN, RUBAIYA Channa striata, commonly known as the striped snakehead, is a high-value freshwater species with significant aquaculture potential in Bangladesh and Southeast Asia. However, its natural populations in Bangladesh are declining due to habitat degradation, overexploitation, and inadequate aquaculture practices. Despite its commercial importance, large-scale seed production remains underdeveloped owing to critical gaps in species identification, reproductive management, induced breeding, and larval rearing protocols.This dissertation aims to bridge these gaps and establish a scientific basis for the sustainable aquaculture of C. striata in Bangladesh. Four sequential studies were conducted: (i) species identification and phylogenetic analysis using geometric morphometrics and COI gene-based DNA barcoding; (ii) assessment of growth and reproductive biology of wild populations from the Atrai River system; (iii) standardization of hormonal induction protocols using carp pituitary extract (CPE) and Buserelin; and (iv) evaluation of weaning strategies for hatchery-produced larvae using Artemia, fish paste, and formulated feeds. The results confirmed clear species delineation among native and exotic Channa spp., with phylogenetic divergence supported by both morphometric and molecular data. Wild C. striata exhibited seasonal reproduction, with peak spawning from April to July. Induced breeding trials demonstrated that both CPE (80 mg/kg) and Buserelin (0.80 µg/kg) effectively stimulated spawning. Larval weaning experiments revealed that initial live feeding with Artemia followed by gradual transition to formulated diets ensured high survival and growth, while water quality, particularly ammonia concentration, played a critical role. Overall, this research provides a comprehensive framework for the conservation and aquaculture of C. striata by integrating taxonomic validation, reproductive biology, induced breeding, and larval nutrition. The findings are expected to support the development of standardized hatchery protocols, contribute to wild stock conservation, and promote commercial-scale seed production in Bangladesh. Future research should emphasize selective breeding, advanced larval nutrition, and field-level application of the proposed protocols for long-term sustainability. GENETIC IDENTIFICATION AND INDUCED BREEDING OF THREATENED INDIGENOUS SPECIES, Channa striata (Bloch, 1793) FOR SEED PRODUCTION; A PhD DISSERTATION BY RUBAIYA PERVIN, Registration No: 2205057, Session: 2022, FISHERIES BIOLOGY AND GENETICS, DEPARTMENT OF FISHERIES BIOLOGY AND GENETICS, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; July, 2025. 2025-07-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2110 AN INVESTIGATION ON CARP FATTENING IN NATORE DISTRICT HOSSAIN, MD. SAKOAT A survey was conducted to assess the current status of carp fattening and the socio economic situation of fish farmers in three upazilas of Natore district: Natore Sadar, Singra, and Gurudaspur. The study took place from July 2022 to June 2023 and involved gathering information from 80 fish farmers through interview with structured questionnaires. Results showed that 40% farmers were within the 41–50 age groups, with 65% belonging to nuclear families and 35% to joint families. In terms of education, most farmers (30%) had secondary-level education, while only 14.5% were illiterate. The annual income of a significant portion of respondents ranged from BDT 1,00,000 to 2,00,000, making up 33.34% of the sample. All farmers had access to electricity, and 41.67% were members of organizations like ASA and BRAC. The study found that the majority of ponds (58.5%) in the study area were between 0.5-1.0 acres in size, with an average depth of 1.5 meter. Additionally, 70% of the ponds used for fish culture were owned by the farmers themselves, while the remaining 30% were leased. Groundwater was the primary water source for 78% of farmers, with 42% exchanging water as needed. Most farmers (57.63%) prepared their ponds and applied specific amounts of salt, TSP, and urea per hectare. Growth promoters were used by 88% of farmers to enhance carp growth, with various probiotics such as Safe gut, Navio plus, Protox aqua, Aquaphoto, and Dellomax being employed. Indian major carp and exotic carp were the preferred species for cultivation, experiencing only fish lice as a constraint during the nine to ten months of cultivation. 63.33% farmers used commercial feeds. The main obstacles identified were a lack of scientific knowledge, insufficient high-quality seeds and feeds, funding shortage and inadequate marketing facilities. AN INVESTIGATION ON CARP FATTENING IN NATORE DISTRICT; A Thesis By MD. SAKOAT HOSSAIN, Examination Roll No. 1606045, Session: 2022, Semester: July-December 2024; MASTER OF SCIENCE (MS) IN AQUACULTURE, DEPARTMENT OF AQUACULTURE, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; DECEMBER 2024. 2024-12-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2109 DETECTION OF MICROPLASTIC FROM THREE IMPORTANT CRAB SPECIES FROM THE COAST OF BANGLADESH HASAN, MD. MEHEDI In this study, the abundances and characteristics of microplastics in different tissues of three species of wild crabs (Scylla olivacea, Portunus pelagicus, and Portonus sanguinolentus) were investigated across three important fishing grounds (Chattagram, Cox’s Bazar and Saint Martin Island region) of Bangladesh. Each region consists of three distinct fishing grounds. The result of the study showed that the highest detection of microplastics was recorded in Cox’s Bazar followed by Saint Martin and Chattagram. The crab species Scylla olivacea and Portunus pelagicus were most abundant in Cox’s Bazar and Chattagram while Portonus sanguinolentus in Cox’s Bazar and Saint Martin Island. The microplastics size was also varied among the crab samples. Comparatively larger microplastics were recorded in the crab samples in Chattagram region. Black coloured microplastics were the most prominent in gill and guts of the samples (45.45% and 42.37%, respectively). Among various types of shapes about (56.92%) of thread was most abundance in gill where thread was (53.33%) in gut. The 50% gill tissue showed fragment microplastics followed by microbead (6.25%). The 49.18% gut tissue showed microbead microplastics followed by fibre (8.20%). Overall, the study reported that the distribution pattern, abundance, size, color and shape of microplastics in the studied crab samples which will ultimately help the biologist to get insight into the plastic pollution in marine ecosystems by taking crabs as model species. DETECTION OF MICROPLASTIC FROM THREE IMPORTANT CRAB SPECIES FROM THE COAST OF BANGLADESH; A THESIS BY MD. MEHEDI HASAN, Registration No. 1706101, MS Session: 2022, Thesis Semester: January - June 2024; MASTER OF SCIENCE (M.S.) IN AQUACULTURE, DEPARTMENT OF AQUACULTURE, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR; JUNE 2024. 2024-06-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2108 AN ASSESSMENT OF CURRENT STATE AND FUTURE POTENTIAL OF BIOFLOC TECHNOLOGY IN PANCHAGARH DISTRICT RAHMAN, MD. HABIBUR The present study was conducted to know the current state and future potential of biofloc technology in the Panchagarh district that cover five upazilas such as Panchagarh sadar, Tentulia, Debiganj, Boda and Atwari starting from July to December 2022. The study was done by analyzing socio-demographic characteristics, farming practices, and challenges faced by local farmers. Data were collected from 55 biofloc farmers through surveys and interviews. The findings revealed that 62.5% of respondents were young farmers aged between 21-30 years, with 40.5% having graduated from formal education systems. The majority (80.58%) hailed from nuclear families, while 74.54% did not hold any organizational membership. Fish culture (30.58%) and biofloc farming (20.32%) were identified as major occupations. Biofloc culture was predominantly practiced by farmers with 7 to 12 months of experience (51.58%), although 70.35% lacked formal training before initiating farming. Most farms (56.36%) operated on areas less than one decimal, with 55.58% having one or two tanks constructed primarily from cement (83.63%). Groundwater was the main water source (92.48%), and partial water exchange practices were widely adopted. Farmers used synthetic probiotics such as Pond Care (40.8%), Protox Aqua (22.81%), Aqua photo (17.32%), Dellomax (10.94%) and Ecopond (6.13%). The preferred tank shapes were rectangular (50.85%) and circular (35.6%), with a depth ranging between 4 to 6 feet. Despite challenges such as technical (50%) and financial (22.75%) difficulties, biofloc farmers reported fish production rates of 301-400 kg per cycle in 38.12% of cases. Diseases, particularly fungal (45.9%) and bacterial (34.5%), posed significant threats. Total harvesting was preferred by 61.82% of farmers, and most sales (52.72%) were made to wholesalers. The findings highlight the promising but challenging adoption of biofloc technology in the district. Investments in farmer training, disease management, and financial support can foster sustainable aquaculture practices and enhance productivity in Panchagarh. AN ASSESSMENT OF CURRENT STATE AND FUTURE POTENTIAL OF BIOFLOC TECHNOLOGY IN PANCHAGARH DISTRICT; A Thesis By MD. HABIBUR RAHMAN, Examination Roll No. 1506109, Session: 2022, Semester: July-December 2024; MASTER OF SCIENCE (MS) IN AQUACULTURE, DEPARTMENT OF AQUACULTURE, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; DECEMBER 2024. 2024-12-01T00:00:00Z