Dept. of Pathology & Parasitology

INVESTIGATION OF CANNABIS EXTRACT AS A POSSIBLE CANDIDATE FOR THE PREVENTION AND TREATMENT OF INDUCE CANCER CELLS IN ALBINO MICE

2023-05-01T00:00:00Z

INVESTIGATION OF CANNABIS EXTRACT AS A POSSIBLE CANDIDATE FOR THE PREVENTION AND TREATMENT OF INDUCE CANCER CELLS IN ALBINO MICE IMAM, MD. RASHED Cancer is the second highest cause of mortality in the world, characterized by the uncontrolled growth and proliferation of cells resulting in death. Cannabinoids are naturally occurring compounds found in the Cannabis sativa plant. Cannabinoids inhibit angiogenesis and decrease metastasis in various tumors. The research work was conducted to evaluate the effects of cannabis extract on albino mice and the response of cannabis extract for the treatment and prevention of Ehrlich ascites carcinoma (EAC) cells induced cancer on albino mice. A total of 444 albino mice were collected from the animal house, at the Department of Biochemistry and Molecular Biology, University of Rajshahi. For the observation of the effects of cannabis extract on albino mice, 48 mice were randomly divided into four treatment groups i.e. T0 (control), T1 (5mg/Kg), T2 (10mg/Kg) and T3 (15mg/Kg). Every week 4 mice were sacrificed from all groups, blood was collected for biochemical and hematological analysis, and organs were collected for histopathological observation. Significantly (P<0.05) highest body weight was found in T3. No significant (P>0.05) effects of cannabis extract on hematological and biochemical parameters except in early-stage SGPT, SGOT, and alkaline phosphatase which were increased but with time it became normal and histopathology of different organs revealed no changes. For the treatment purpose of EAC-induced cancer, 204 mice were randomly divided into seven groups i.e group-1 (control, n=12) group-2 (EAC induced only, n=12), group-3 (Commercial Chemotherapeutic drug, n=36), group-4 (cannabis extract started injection 3 days after EAC induced, n=36), group-5 (cannabis extract started injection 7 days after EAC induced, n=36), group-6 (cannabis extract started injection 14 days after EAC induced, n=36) and group-7 (cannabis extract started injection 21 days after EAC induced, n=36). Mice of group-3, group-4, group-5 group-6 and group-7 were divided into four subgroups i.e T0 (control), T1 (5mg/Kg), T2 (10mg/Kg) and T3 (15mg/Kg) consisting of 12 mice in each. The results revealed that no tumor and ascitic fluid formation and no significant (P>0.05) effects on biochemical and hematological parameters in the early period of treatment (groups 4 and 5) whereas tumor and acetic fluid formation and had significant (P>0.05) effects on some biochemical and hematological parameters group at in the longer period of treatment (group 6 and 7), cytopathological study of ascitic fluids also revealed atypical cells under light microscope, histopathological study of tumor revealed sarcoma type cancer. For the prevention purpose of EAC-induced cancer, a total of 192 mice were randomly divided into four groups i.e group-1 (EAC induced after 3 doses of cannabis extract, n=48), group-2 (EAC induced after 7 doses of cannabis extract, n=48), group-3 (EAC induced after 14 doses of cannabis extract, n=48) and group-4 (EAC induced after 21 doses of cannabis extract, n=18). Mice of group-1, group-2, group-3 and group-4 were divided into four subgroups i.e T0 (control), T1 (5mg/Kg), T2 (10mg/Kg) and T3 (15mg/Kg) consisting of 12 mice in each. The effects of EAC cells induced cancer and preventive effects of cannabis extract on biochemical and hematological profiles were observed. In the biochemical profile, EAC significantly (P<0.05) increased SGPT, SGOT, alkaline phosphate and uric acid with an increased period of observation. EAC significantly (P<0.05) increased WBC and neutrophil but significantly (P<0.05) decreased lymphocyte. After 3rd and 7th doses of extract injection did not show cancer preventive effect. After the 14th dose, 7.14% tumor and fluid formation, no significant effect (p<0.05) on biochemical and hematological parameters except SGPT, SGOT and alkaline phosphatase. EAC induced after 21st doses of cannabis extract injection, no tumor and acetic fluid formation, no significant effect on biochemical and hematological parameters like as normal mice. INVESTIGATION OF CANNABIS EXTRACT AS A POSSIBLE CANDIDATE FOR THE PREVENTION AND TREATMENT OF INDUCE CANCER CELLS IN ALBINO MICE; A Ph.D. DISSERTATION BY MD. RASHED IMAM, Registration No. 120508, DEPARTMENT OF PATHOLOGY AND PARASITOLOG, FACULTY OF VETERINARY AND ANIMAL SCIENCE, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY, UNIVERSITY, DINAJPUR-5200; May, 2023.

CLINICOPATHOLOGICAL STATUS OF DUCK PLAGUE AT DINAJPUR DISTRICT

2014-06-01T00:00:00Z

CLINICOPATHOLOGICAL STATUS OF DUCK PLAGUE AT DINAJPUR DISTRICT MOSTARI, SHABNAM Clinicopathological status of duck plague was investigated at different upazila of Dinajpur district during the period from January to June, 2014. The clinical features emphasiging the mortality and prevalence, necropsy for gross morbid lesions, histopathological features were examined. The farm and flock history, managemental aspects, vaccination status, nutrition, etc. were recorded carefully. The data was collected and statistically analysed. Farmer’s complaint’s about their affected birds were also considered and emphasized. The average mortality rate was recorded as 14.42% and prevalence was 19.81%. The sick birds clinically showed moderate to severe depression, ocular and nasal discharges, ataxia, dypsnia. The affected organs were pathologically characterized as mild to moderate congestion and haemorrhages; misshapen, ruptured cystic ova, and histopathologically characterized as mild to moderate architectural destruction, reactive cell infiltration. A Thesis By SHABNAM MOSTARI Registration No. 1305075 Semester: January- June, 2014 Session: 2013-2014 Submitted to the Department of Pathology and Parasitology Hajee Mohammad Danesh Science and Technology University, Dinajpur, In partial fulfillment of the requirements for the degree of Master of Science (M.S.) in Pathology Department of Pathology and Parasitology

INVESTIGATION OF DIFFERENT PARASITES OF FARMED PIGEONS AT DINAJPUR SADAR UPAZILA

2017-06-01T00:00:00Z

INVESTIGATION OF DIFFERENT PARASITES OF FARMED PIGEONS AT DINAJPUR SADAR UPAZILA RAHMAN, MD. GAUSUR A cross sectional study was conducted to investigate the different parasites in farmed pigeon at sadar upazila of Dinajpur, Bangladesh during January to June, 2017. A total of 122 pigeons (39 young and 83 adult in which 45 male and 77 female) from different farms were examined by faecal, oral and blood sample examination and postmortem examination for histopathological study. The investigation expressed that the highest overall prevalence was ectoparasites 107 (87.70%) followed by helminthes 96 (78.69%) and protozoa 71(58.20%). In this study, the frequency of Echinostoma sp., Raillietina sp., Ascaridia sp., Capillaria sp., Ornithostrongylus sp., Eimeria sp., Trichomonas sp., Haemoproteus sp., Columbicola columbae, Menopon sp. and Lipeurus sp. were found to be 24(19.67%), 46(37.67%), 51(41.80%), 38(31.15%), 15(12.30%), 45(36.89%), 47(38.52%), 39(31.97%), 96(78.67%), 55(45.08%) and 75(61.48%) respectively. The age and sex related prevalence of helminth revealed that adults 68(81.93%) were more susceptible than young 28(71.79%) and females 62(80.52%) were more prevalent than male 34(75.56%) but these were statistically insignificant (P>0.05). Further, youngs 25(64.10%) were more prone to protozoa than adults 46(55.42%) and the higher prevalence of protozoa was found in female 50(64.94%) in compare to male 21(46.67%) which were not statistically significant (P>0.05). Association of age and sex with ectoparasites indicated that the prevalence of ectoparasites was significantly (P<0.001) higher in adult 80(96.39%) than young 27(69.23%) and also significantly (P<0.05) higher in female 50(64.94%) than male 21(46.67%) pigeons. The mean intensity of Echinostoma sp., Raillietina sp., Ascaridia sp., Capillaria sp., Ornithostrongylus sp., Eimeria sp., Trichomonas sp., Haemoproteus sp., Columbicola columbae, Menopon sp. and Lipeurus sp. was 2.50±0.30, 4.30±0.35, 3.80±0.29, 2.53±0.23, 1.92±0.24, 4.38±0.38, 4.53±0.42, 7.82±0.61, 17.32±0.82, 14.47±1.13 and 11.91±0.85 respectively. Histopathologically found that degeneration, distraction and desquamation of papillae, villi and epithelium of intestine. The results indicate that pigeons of this area are very much susceptible to different endo and ectoparasites which cause great economic loss of the farmer. A Thesis By MD. GAUSUR RAHMAN Student ID. 1605142 Session: 2016-2017 Semester: January-June, 2017 MASTER OF SCIENCE (M.S.) IN PARASITOLOGY

PREVALENCE OF TICK INFESTATION IN CATTLE AT SADAR UPZILA OF DINAJPUR DISTRICT

2017-06-01T00:00:00Z

PREVALENCE OF TICK INFESTATION IN CATTLE AT SADAR UPZILA OF DINAJPUR DISTRICT ROZARIO, DIPA The study was designed with an aim to determine the overall prevalence of tick infestation in relation to age, sex, breed, health status, season, management system and affected body parts of the cattle at Sadar upzilla of Dinajpur district during the period from January to June, 2017. The investigation was done by collecting ticks with fine forceps and identifying ticks by preparing temporary and permanent slides. A total of 140 cattle (male 45 and female 95) were examined. Among them, 62 (44.29%) were found to be infested with three species of ticks namely Rhipicephalus sanguineus, Boophilus microplus and Hemaphysalis bispinosa. The prevalence of tick was higher in case of Rhipicephalus sanguineus (27.14%) followed by Boophilus microplus (15.71%) and Hemaphysalis bispinosa (1.43%). The results revealed that the prevalence was significantly (P<0.05) higher in female cattle (52.63%) than in male (26.67%). Malnourished cattle were vulnerable (51.85%) to tick infestation than the cattle with normal body condition (33.90%). In age groups, highest infestation was found in calf (< 6 months) (58.33%) followed by adults (> 2 years) (47.05%) and the lowest in young (≤ 2 years) (33.33%). Tick infestation was more prevalent in local (46.24%) cattle than the cross-bred (40.43%) cattle. In case of cattle management system significantly (p<0.005) higher prevalence was observed in cattle reared under extensive system (65.38%) than the intensive (47.37%) and semi-intensive (34.21%) systems. Prevalence was significantly (p<0.001) higher in rainy (54.17%) season than summer (33.82%) season. Ticks were distributed in different parts of the host body such as ear, neck, dewlap, base of horn, eye, tail, perineum, udder and body surface. Among the body parts, ear (35.71%) was the most affected part and tail (3.71%) was the least. It is concluded that tick infestation is a threat to the cattle population at Sadar upzilla of Dinajpur irrespective of age, sex, breed, health status, seasons of the year, management system and affected body parts. This might hamper cattle production in this area. Hence attention in cattle management and appropriate control strategies are need to be initiated to control ticks in the study area. A Thesis By DIPA ROZARIO Student ID. 1605143 Session: 2016-2017 Semester: January-June, 2017 MASTER OF SCIENCE (MS) IN PARASITOLOGY