Dept. of Microbiology

INVESTIGATION ON FOWL ADENOVIRUS (GROUP 1) CIRCULATING IN BANGLADESH AND MOLECULAR CHARACTERIZATION OF SPECIFIC SEROTYPES ASSOCIATED WITH INCLUSION BODY HEPATITIS

2024-06-01T00:00:00Z

INVESTIGATION ON FOWL ADENOVIRUS (GROUP 1) CIRCULATING IN BANGLADESH AND MOLECULAR CHARACTERIZATION OF SPECIFIC SEROTYPES ASSOCIATED WITH INCLUSION BODY HEPATITIS Islam, Mohammad Nazrul Fowl adenoviruses (FAdVs) cause numerous diseases resulting in economic losses to the poultry industry worldwide. Several FAdV serotypes cause inclusion body hepatitis in chicken. Although FAdV infection was suspected, there was no confirmatory report from Bangladesh. The study was conducted to investigate the FAdV infection and specific antibodies in chicken. A total of 50 samples, each composed of liver and spleen, were collected from different chickens of Gazipur, Dinajpur and Panchagarh district. Three suspected organ samples derived from liver were also inoculated in to SPF embryonated chicken eggs for viral propagation. Each location is belonging to A, B and C poultry zones of Bangladesh, respectively. Viruses were detected by real-time PCR and conventional PCR. Blood samples (n=303) were collected at beginning and after recovery from infection and tested by indirect ELISA. Sequencing of PCR products were done for phylogenetic analysis. The indirect ELISA serological technique was employed which detects antibodies to Group 1 FAdVs and does not differentiate specific serotypes. Specific serotypes were detected through real time PCR and sequencing data analysis. All the flocks exhibited clinical symptoms of anorexia, drowsiness, ruffled feathers, reduced body weight, lack of uniformity and high mortality. Enlarged friable liver with yellow to tan color mottled with focal soft area, fluid in pericardial sac, swollen and hemorrhagic kidneys, enlarged congested spleen and hemorrhagic pancreas were found on postmortem examination. FAdVs were detected in 9 flocks (90%) out of 10 flocks except commercial layer flock from Dinajpur. Three serotypes namely serotype 8b in 7flocks (70%), serotype 11 in 2 flocks (20%) and serotype 5 in 1 flock (10%) were detected. The antibody titer increases significantly (p<0.05) after recovery from the infection. Phylogenetic analysis revealed that the Bangladeshi FAdVs have close identity with viruses from Asia, Europe, South and North America. These results implied that FAdVs have been introduced to Bangladesh multiple times. In this situation vaccination against detected particular serotypes associated with IBH (Inclusion Body Hepatitis) and upholding comprehensive biosecurity measures are crucial for the prevention and control of the disease. INVESTIGATION ON FOWL ADENOVIRUS (GROUP 1) CIRCULATING IN BANGLADESH AND MOLECULAR CHARACTERIZATION OF SPECIFIC SEROTYPES ASSOCIATED WITH INCLUSION BODY HEPATITIS; A Dissertation Submitted By Mohammad Nazrul Islam, Registration No.: 1205017, Session: 2020; In partial fulfillment of the requirements for the degree of Doctor of Philosophy In Microbiology, DEPARTMENT OF MICROBIOLOGY, FACULTY OF VETERINARY AND ANIMAL SCIENCE, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR; June, 2024.

Circulating Tumor Cell Chip in Breast Cancer for Theragnosis based on Surface—Enhanced Raman Spectroscopy

2014-01-01T00:00:00Z

Circulating Tumor Cell Chip in Breast Cancer for Theragnosis based on Surface—Enhanced Raman Spectroscopy Hossain, Md. Khaled Circulating Tumor Cell Chip in Breast Cancer for Theragnosis based on Surface-Enhanced Raman Spectroscopy Dissertation Director: Professor Jeong-Woo Choi Circulating tumor cells (CTCs) are emerging biomarkers, especially in case of liquid biopsy, and important indicator for prognosis monitoring in case of personalized anticancer therapy. CTC analysis is a promising diagnostic method for estimating the risk of metastatic relapse and metastatic progression in patient with cancer. The basic problem of CTC study is their extremely low inherent numbers in blood (around one CTC per 10° non cancerous hematopoietic cells). Hence, before detection or characterization of CTCs their isolation is important. A subpopulation of CTCs with stemlike behavior are known as stem-like circulating tumor cells (SCTCs). In recent years stem like cancer cells (SCCs) hypothesis has attracted great attention in the field of cancer biology. According to the concept, a minor Circulating Tumor Cell Chip in Breast Cancer for Theragnosis based on Surface—Enhanced Raman Spectroscopy Md. Khaled Hossain

ISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICTISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICT

2019-12-01T00:00:00Z

ISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICTISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICT SALAD, SAID MOHAMED The present research work was under taken to isolate and identify the associated bacteria of chicken raw meat and to find out the effective antibiotics against the bacteria through antibiogram studies in Dinajpur district Bangladesh. July to November, 2019. A total of 48 samples were randomly collected from markets and transported to the Microbiology laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur. After processing of samples primary culture was done in nutrient broth and nutrient agar then pure culture was obtained from different selective media. The level of bacterial load were significantly higher the first sampling in comparison with the second and third one respectively. The prevalence of Escherichia coli, Salmonella spp. and Staphylococcus spp. were 47.9%, 39.6% and 12.5% respectively. All pure isolates were subjected to Antibiogram assay test by disc diffusion method against 8 different antibiotics. E. coli isolates were sensitive moxifloxacin. Highest resistant to chloraphenicol, tetracycline and streptomycin. Among all Salmonella spp., isolates, chloraphenicol showed the highest susceptibility pattern followed by the tetracycline, azithromycin, ampicillin and Erythromycin. Highest resistant pattern was showed by penicillin, Cefalexin and amoxicillin. In case of Staphylococcus spp., chloraphenicol showed the highest susceptibility pattern followed by the streptomycin and streptomycin found sensitive in this study. Highest resistant pattern showed by the tetracycline, azithromycin, ampicillin and tetracycline. This study revealed that broiler meat sold at some local markets in Dinajpur city were contaminated with multiple species of multidrug resistant bacteria which may risk for human health. A THESIS BY SAID MOHAMED SALAD REGISTRATION NO. 1805501 SEMESTER: JULY-DECEMBER, 2019 SESSION: 2018 Submitted to the Department of Microbiology Hajee Mohammad Danesh Science and Technology University, Dinajpur In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (M.S.) IN VETERINARY PUBLIC HEALTH AND FOOD HYGIENE

DETEMINATION OF MICROBIAL QUALITY OF DRINKING WATER OBTAINED FROM DIFFERENT SOURCES FOR HUMAN, POULTRY AND DAIRY FARM IN DINAJPUR DISTRICT OF BANGLADESH

2019-06-01T00:00:00Z

DETEMINATION OF MICROBIAL QUALITY OF DRINKING WATER OBTAINED FROM DIFFERENT SOURCES FOR HUMAN, POULTRY AND DAIRY FARM IN DINAJPUR DISTRICT OF BANGLADESH ROY, MITHUN CHANDRA Total ninety (90) samples, thirty 30 from human tubewell water, 30 water samples of dairy farm and 30 water samples of poultry farm were collected and tested for TVC and MPN to to determine the microbial quality of drinking water. The mean HPC of human tubewell water were found 2.55×103 , 3.11×103 , 2.85×103 , 2.88×103 and 3.20×103 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest HPC was found in Chirirbandar 3.20×103 CFU mL-1 and lowest found in Sadar 2.55×103 CFU mL-1. The MPN values were found 2.16 , 2.16 , 2.50 , 1.83 and 2.33 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest MPN value was found in Kaharol 2.50 and lowest were found in sadar 2.16 and Chirirbandar 2.16 coliforms/100 ml .The mean HPC of dairy farm tubewell water were found 2.3×103 , 3.6×103 , 2.7×103 , 3.2×103 and 3.4×103 CFU mL-1 in Sadar, Birgonj, Kaharol ,Birol and Chirirbandar .The highest HPC of dairy farm tubewell water was found in Birgonj 3.6×103 CFU mL-1 and lowest HPC was found in Sadar 2.3×103 CFU mL-1. The MPN values were found 1.66, 2.33, 2.66, 2.00 and 2.66 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The highest MPN values were found in Kaharol 2.66 coliforms/100 ml and Chirirbandar 2.66 coliforms /100 ml and lowest was found in sadar 1.66 coliforms/100 ml of water.The mean of HPC of dairy farm manger water were in 4.66×107 , 2.0×107 , l 2.8×107 , 2.8×107 and 4.2×107 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest mean HPC of dairy farm manger water was found in Sadar 4.66×107 CFU mL-1 and lowest found in Birgonj 2.0×107 CFU mL-1 .The MPN values were found 14.00, 17.33, 14.00, 16.33 and 15.33 coliforms /100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The MPN of dairy farm manger water was highest in Birgonj 17.33 coliforms /100 ml and lowest were found in Sadar 14.00 coliforms /100 ml and Kaharol 14.00 coliforms/100 ml.The geometric mean of HPC of Poultry farm (Tubewel/Machine) water were Sadar 2.8×103 CFU mL-1 , Birgonj 2.67×103 CFU mL-1 , Kaharol 3.0×103 CFU mL-1 , Birol 2.57×103 CFU mL-1 and Chirirbandar 3.0×103 CFU mL-1 .In this study, it was found that HPC of Poultry farm tubewell water was highest in Kaharol 3.0×103 CFU mL-1 and lowest in Birol 2.57×103 CFU mL-1 .The MPN values of Poultry farm tubewell water were found 2.66, 2.00, 2.33, 1.66 and 2.00 coliforms/100 ml of drinking water in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The MPN of Poultry farm tubewell water was highest in Sadar 2.66 coliforms/100 ml and lowest in Birol 1.66 coliforms/100 ml.The mean of HPC of Poultry farm waterer water were found 4.2×107 , 2.00×107 , 3.25×107 , 1.00×107 and 2.00×107 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. Highest HPC of Poultry farm waterer water was found in Sadar 4.2×107 CFU mL-1 and lowest in Birol 1.00×107 CFU mL-1 . The MPN values of poultry waterer water were 12.33, 9.66, 10.33, 13.33 and 12.33 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The highest MPN value was found in Birol 13.33 coliforms/100 ml and lowest were found in Birol 9.66 coliforms/100 ml. From ninety water samples seven bacteria were isolated among them the most frequent isolate was Escherichia coli 82(15.95%) followed by Salmonella spp 81(15.75%), Shigella spp 80(15.56%), Klebsiella spp 79(15.36%), Vibrio spp 73(14.20%), Pseudomonas spp 60(11.67%) and Staphylococcus spp 59(11.47%). The antimicrobial susceptibility pattern showed that the isolates were highly resistant to ampicillin, amoxicillin, erythromycin and chloramphenicol and susceptible to gentamicin, azithromycin, colistin, ceftriaxone, levofloxacin and ciprofloxacin. Out of seven bacteria two bacteria (Escherichia coli and Vibrio spp) are subjected to amplified by using 16S rRNA gene based PCR A THESIS BY MITHUN CHANDRA ROY REGISTRATION NO. 1705432 SEMESTER: JANUARY-JUNE, 2019 SESSION: 2017 Submitted to the Department of Microbiology Hajee Mohammad Danesh Science and Technology University, Dinajpur In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (M.S.) IN Veterinary Public Health and Food Hygiene