Dept. of Biochemistry & Molecular Biology

Impact of chlorpyrifos degrading consortia on agronomic yields, heavy metals and minerals parameter of BRRI Dhan 28

2023-06-01T00:00:00Z

Impact of chlorpyrifos degrading consortia on agronomic yields, heavy metals and minerals parameter of BRRI Dhan 28 Hossain, Emarat Citrobacter sp. HSTU-ABk15, Acinetobacter sp. HSTU-ASm16, and Serattia sp. HSTU-ABk35 are three endophytic bacteria which have significant effect on plant growth and yield promotion and also have pesticide degrading capability – these are proved previously through molecular and genomics identification in dry laboratory. Therefore, this study is designed for field experiment of these three strains on rice plants in selected BIRI 28 verities in Dinajpur, Bangladesh region. Field experiment was designed to give treatment on various groups are- 30%, 40% and, 50% urea with single bacteria and also mix consortium of bacteria was given as treatment 30%40% and 50% urea respectively. In 16 different plots which area were divided in 1*0.76 m. thirty days aged plants were planted and three times rice plants were collected from field in every fifteen days to quantify Growth and yield parameters with other physical properties. And after cultivation of rice, I performed biochemical and minerals test such as – b carotene, vitamin- c and ca, zn to find out the rice quality regarding to control. The maximum amount of calcium, Magnesium, Boron, Copper, Lead concentration was observed in 40%mix treatment. There is no significant difference for protein content among the treatments but Carbohydrate and food energy content was higher where no bacterial treatment. Insignificant difference was found among all the treatments for Cadmium accumulation. Finally, the most significant and desirable yield without health hazards was found on 30% mix treatments which is 1.57-fold higher than the farmers practices where full doses of recommended fertilizers were used having the heavier materials more than the acceptance. Impact of chlorpyrifos degrading consortia on agronomic yields, heavy metals and minerals parameter of BRRI Dhan 28; A THESIS BYEmarat Hossain, Student ID: 2105022, Session: 2021-2022; MASTER OF SCIENCE (M.S.) IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; JUNE,2023.

Unveiling the Multifaceted Potential of Acinetobacter sp. strain HSTU BF2R3 to Promote Tomato Plant Growth: A Comprehensive Genomic Analysis of Endophytic Bacterium Isolated from Eggplant Root

2023-06-01T00:00:00Z

Unveiling the Multifaceted Potential of Acinetobacter sp. strain HSTU BF2R3 to Promote Tomato Plant Growth: A Comprehensive Genomic Analysis of Endophytic Bacterium Isolated from Eggplant Root KAIUM, ABDUL AL Acinetobacter sp. strain HSTU-BF2R3, a novel endophytic bacterium, was isolated from the roots of eggplant (Solanum melongena) near the campus of HSTU (Hajee Mohammad Danesh Sciences and Technology University). The complete genome sequence of this bacterium was analyzed, revealing the presence of various genes associated with plant growth promotion and pesticide degradation. By performing functional annotation and comparative genomic analysis, we uncovered a plethora of endophytic bacterium genes in Acinetobacter sp. HSTU-BF2R3 that contribute to plant growth promotion. The aims of this study are to point out the evaluation of biochemical and plant growth promoting characteristics, whole genome sequencing, and pesticide metabolites capability by the isolated strain. The isolated strain showed positive and negative results in different biochemical tests according to its nature. The isolate has shown suitable in vitro growth promoting activities between different groups of treatment and was exhibited very significant growth-promoting effects compared with the control on Tomato plants and significant (p< 0.05) difference have been revealed in plant growth parameters between treatments. In this research paper, we conducted an in-depth analysis of the HSTU BF2R3 strain genome to elucidate its plant growth-promoting capabilities, with a focus on the identification of various genes involved in nitrogen fixation, indole-3-acetic acid (IAA) production,1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, sulfur metabolism, phosphate metabolism, and other growth-promoting genes. The identified genes could play a vital role in enhancing plant growth and protecting the plant from harmful pesticide residues. The phylogenetic tree analysis was constructed based on conserved genomic markers to position HSTU-BF2R3 within the Acinetobacter genus and elucidate its evolutionary relationship with closely related strains. Through this analysis, we sought to understand its phylogenetic placement and evolutionary divergence. The findings from this study revealed that Acinetobacter sp. HSTU-BF2R3 possesses a unique genomic profile with a rich repertoire of genes involved in plant growth promotion and pesticide degradation. This suggests its potential application as a biofertilizer and bioremediation agent for sustainable agriculture practices. Moreover, the phylogenetic analysis indicated its distinct evolutionary history within the Acinetobacter genus, possibly signifying its adaptation to the plant rhizosphere. The genomic insights provided in this study offer a solid foundation for further research and exploration of this promising bacterium's agricultural applications, which could ultimately contribute to the development of eco-friendly and sustainable crop management strategies. Unveiling the Multifaceted Potential of Acinetobacter sp. strain HSTU BF2R3 to Promote Tomato Plant Growth: A Comprehensive Genomic Analysis of Endophytic Bacterium Isolated from Eggplant Root; A THESIS BY ABDUL AL KAIUM, Student ID: 2205038, Session: 2022-2023, MASTER OF SCIENCE (M.S.) IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; JUNE 2023.

FOLIAR APPLICATION OF BORON AND ZINC ON GROWTH YIELD AND BIOCHEMICAL PARAMETERS OF COMMON BUCKWHEAT

2023-12-01T00:00:00Z

FOLIAR APPLICATION OF BORON AND ZINC ON GROWTH YIELD AND BIOCHEMICAL PARAMETERS OF COMMON BUCKWHEAT MOHIBBULLAH, MD. AL Common buckwheat (Fagopyrum esculentum Moench) is a pseudocereal that is widely cultivated for its nutritional and medicinal properties. boron and zinc are essential elements for plant growth, affecting yield, and seed production. A field experiment was conducted at the biochemistry research field of Hajee Mohammad Danesh Science and Technology University in Dinajpur during the winter season from december 2022 to april 2023. The objective of the experiment was to investigate the impact of foliar application of boron and zinc on the growth, yield, and biochemical parameters of common buckwheat. The treatments included zinc (7 kg/h-1) and boron (2 kg/h-1), while no sprays were used as a control. The experiment was designed based on a randomized complete block design (RCBD) with three replications. The seed was applied at a rate of 30 kg/ha-1, and the recommended dose of urea, triple super phosphate (TSP), and muriate of potash (MOP) was applied at a rate of 65, 85, and 35 kg/ha-1, respectively. Results of the experiments showed that foliar application of zinc and boron in common buckwheat resulted in significant variation for several important parameters. Our study found that foliar application of zinc of common buckwheat resulted in a significant increase in plant height (90cm), number of sub-branches (18), However, the application of both zinc and boron through foliar spraying led to a significant increase in the, number of grain per raceme (9), grain settings raceme per plant (46), total number of grain per plant (224), thousand grain weight (16.88), seed weight per plot (486g), compared to the control group. Furthermore, both boron and zinc treatments enhanced the activities of ascorbate peroxidase (APX), peroxidase (POD), and catalase (CAT) in common buckwheat, while hydrogen peroxide (H2O2) activity decreased. The utilization of various microelements, including boron and zinc, has the potential to enhance the yield and quality of common buckwheat. Moreover, the application of zinc and boron through foliar spraying can be beneficial in increasing seed set and quality, especially in regions with arid and semi-arid climates. FOLIAR APPLICATION OF BORON AND ZINC ON GROWTH YIELD AND BIOCHEMICAL PARAMETERS OF COMMON BUCKWHEAT; A THESIS BY MD. AL MOHIBBULLAH, Registration No.: 2205141, Session: 2022-2023; MASTER OF SCIENCE (MS) IBIOCHEMISTRY AND MOLECULAR BIOLOGY, DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; DECEMBER, 2023.

EFFECT OF SEED RATES ON GROWTH YIELDS AND BIOCHEMICALS PARAMETER OF BUCKWHEAT

2023-12-01T00:00:00Z

EFFECT OF SEED RATES ON GROWTH YIELDS AND BIOCHEMICALS PARAMETER OF BUCKWHEAT UDDIN, MD. MOSLEM Buckwheat is a medicinal plant whose seedlings contain numerous vitamin C and grains that have complete protein and other micro and macronutrients. The study was carried out to investigate the effect of seeding rate on growth, yield and biochemical parameters of buckwheat and to find out the suitable seeding rate for the farmer’s field. The experiment was divided into four groups viz 10 (T1), 20 (T2), 30 (T0), 40 (T3), and 50 (T4) kg ha-1 in randomized complete block design (RCBD) with three replications. Yield growth characteristics like plant height (cm), branches and sub-branches per plant, root length (cm), number of inflorescences and number of clusters per plant, number of grains per cluster, grain yield per plot, etc. were significantly affected by seed rates. A low seeding rate results in increased uptake of light, water, air, nutrition, and NPK. Due to the higher uptake of NPK in low-seed plants, they had higher activities of antioxidant enzymes than high-seeding rates plants. Plants with low seeding rates may have lower levels of hydrogen peroxide due to increased levels of the hydrogen peroxide scavenging enzymes catalase, peroxidase, and ascorbate peroxidase. Low-seeded plants have high-yield traits, but optimum seeding rates result in higher yields due to optimum plant numbers. The highest grain yield was 648 grams in 20 kg ha-1 seed rate plots (T2) and the lowest was 304 grams in 50 kg ha-1 seed rate plots (T4). A comparison of yields from 20 kg ha 1seed rate (T2) to 30 kg ha-1 (T0) and 40 kg ha-1 (T3) shows that the yield decreased due to yield contributing factors. An optimum seed rate of 20 kg ha-1 (T2) was identified with the highest yield of 648 g per 8-meter square plot. Therefore, a seed rate of 20 kg ha-1 showed a significant effect on growth, yield and the biochemical properties of buckwheat. EFFECT OF SEED RATES ON GROWTH YIELDS AND BIOCHEMICALS PARAMETER OF BUCKWHEAT; A THESIS BY MD. MOSLEM UDDIN, Student ID.: 2205139, Session: 2022-2023; MASTER OF SCIENCE (MS) IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; DECEMBER, 2023.