http://103.7.193.12:8080/xmlui/handle/123456789/183 2026-04-17T15:35:10Z http://103.7.193.12:8080/xmlui/handle/123456789/2092 INTEGRATED MANAGEMENT OF FUSARIUM WILT AND COLLAR ROT OF CHICKPEA Wilt and collar rot of chickpea caused by Fusarium oxysporum f. sp. ciceri and Sclerotium rolfsii, respectively is a serious disease responsible for maximum yield loss in Bangladesh. For integrated management of Fusarium wilt and collar rot of chickpea, Trichoderma asperellum HSTUT 1, Neem leaf extract and Autostin 50 WDS were selected. In dual culture, T. asperellum HSTUT 1 inhibited the mycelial growth of F. oxysporum and S. rolfsii by 63.33 and 56.66 %, respectively. Well diffusion assay showed that neem leaf extract and Autostin 50 WDG also inhibited the mycelial growth of F. oxysporum by 38.88, 65.22, 73.00 & 86.66% and S. rolfsii by 35.55, 60.77, 66.33 & 74.88%, respectively over control. In the field conditions, combined application of T. asperellum HSTUT 1, neem extract and Autostin 50 WDG resulted higher reduction of wilt incidence by 9.66, 1.21 & 0.00 % and collar rot incidence by 9.14, 2.33 & 0.00% at 30, 45 and 60 DAS, respectively in comparison to other treatments. Combined application of Trichoderma, neem leaf extract and Autostin 50 WDG also exhibited the highest germination (84.55 & 90.22%), highest shoot length (18.24 & 16.10 cm), highest root length (5.92 & 7.30 cm), highest shoot weight (0.88 & 0.83g), highest root weight (0.32 & 0.32g), highest pod number per plant (26, 49.33), highest nodule number per plant (46.33 & 31.00) and highest yield per plant (4.16 & 7.89g), respectively in Fusarium wilt and collar rot pathogen infected plant. The finding of the present study explored the potentiality of the combination of T. asperellum HSTUT 1, neem leaf extract and Autostin 50 WDG in the eco-friendly management of Fusarium wilt and collar rot of chickpea in field conditions. INTEGRATED MANAGEMENT OF FUSARIUM WILT AND COLLAR ROT OF CHICKPEA; A THESIS BY Student No: 1701314, Session: 2023-2024; MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY, DEPARTMENT OF PLANT PATHOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; JUNE 2024 . 2024-06-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2088 Enhanced disease resistance in Yard-long bean (Vigna unguiculata) against Brown Rust and Fusarium Wilt diseases using Trichoderma and antioxidants ZULFIKER, ANTARIN Yard-long bean (Vigna unguiculata) is a significant leguminous crop in tropical countries. The vegetable is becoming more and more popular, because of its excellent flavour and high nutritional content. Brown rust and Fusarium wilt disease of yard-long beans caused by Uromyces phaseoli typica and Fusarium oxysporum f. sp. phaseoli are two devastating diseases responsible for maximum yield loss in Bangladesh. The present study was aimed at the bio-control of Brown rust and Fusarium wilt disease as an alternative means of chemical control. For enhancing systemic resistance in yard-long bean plants against Uromyces phaseoli typica and Fusarium oxysporum f. sp. phaseoli as well as for the sustainable and eco-friendly management of Brown rust and Fusarium wilt disease, two previously isolated Trichoderma (T. asperellum and T. harzianum) were used to control these diseases. This study was also concerned with the application of antioxidants; salicylic acid and tartaric acid. In the pot experiment, the triple combination of charcoal-based formulated T. asperellum, salicylic acid (4 mM), and tartaric acid (10 mM) exhibited the highest reduction of Rust incidence (75.02 %) and Rust severity (75.38 %) over control as well as Wilt disease incidence (69.86 %) and Wilt disease severity (80.56 %) over control at 60 days after sowing (DAS). The triple combination of charcoal-based formulated T. asperellum, salicylic acid (4 mM), and tartaric acid (10 mM) resulted in the highest yield in yard long bean (5.3 Kg/plant) compared to other treatments and control. This combination also exhibited the best results in all tested parameters in plant height (199.43 cm), leaf number (60.33), number of pod/plant (84.67) at 90 DAS, and pod length (65.63 cm). Furthermore, this combined application showed maximum total chlorophyll content (151.33 mg/g FW) as well. The study explored the use of charcoal-based formulated T. asperellum, salicylic acid (4 mM), and tartaric acid (10 mM) for the preparation of bio-fertilizer and/or bio-pesticides for the field application to control Brown rust and Fusarium wilt disease of yard-long bean. Enhanced disease resistance in Yard-long bean (Vigna unguiculata) against Brown Rust and Fusarium Wilt diseases using Trichoderma and antioxidants; A THESIS BY ANTARIN ZULFIKER, Student No. 1701185, Session: 2023-2024, Thesis Semester: January-June, 2024; MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY, DEPARTMENT OF PLANT PATHOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR; JUNE, 2024. 2024-06-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2087 Liquid Formulation of Native Trichoderma spp. for the Biocontrol of Fusarium leaf spot of Bottle Gourd OTITHI, SIDRATUL MUNTAHA BINTE ANAM Fusarium leaf spot of Bottle Gourd caused by Fusarium oxysporum is a newly emerging disease in Bangladesh. The present study aimed to develop a Potato-dextrose and yeast molasses-based upgraded Trichoderma liquid formulation for the eco-friendly and sustainable management of the disease. Therefore two native Trichoderma species (Trichoderma HSTUT-6 & Trichoderma HSTUT-8) were used to develop the liquid formulations. In-vitro tests demonstrated the antifungal efficacy of the selected Trichoderma against the isolated and molecular identified Fusarium sp., the causal agent of leaf spot disease. Trichoderma HSTUT-8 showed a maximum zone of inhibition (86.67 %) against F. oxyxporum in the dual culture method followed by Trichoderma HSTUT-6 (82.23 %). Trichoderma HSTUT-8 grown on potato-dextrose-based liquid formulation showed the highest reduction of leaf spot incidence (70.03 % & 73.86 %) and severity (90.19 % and 88.89 %) over control at 80 days after sowing (DAS). This upgraded liquid formulation also showed higher plant height (51.33 & 49 cm), total chlorophyll content (1.88 & 1.84 mg/g fresh weight, respectively), total phenols content in fruits (0.583 & 0.553 mg catechol/100 g fresh weight), number of fruits/plant (13.67 & 14.67, respectively), fruit length (51.33 & 49 cm, respectively), weight (2.24 & 3.093 Kg, respectively), diameter (32.33 & 39.33 cm, respectively), total soluble solids in fruits (6.23 & 5.7 %, respectively), pH (6.8 & 6.64, respectively), moisture content (97.2, 97.63 %) in fruits of Naaz Green and Diana Bottle Gourd. Furthermore, the combined application of potato-dextrose-based Trichoderma liquid formulation showed minimum firmness of fruits (20.53 & 20.33 Kg/m2). The study explored the use of the developed liquid formulation of Trichoderma HSTUT-6 and Trichoderma HSTUT-8 for the field application to control Fusarium leaf spot of Bottle Gourd. Liquid Formulation of Native Trichoderma spp. for the Biocontrol of Fusarium leaf spot of Bottle Gourd; A THESIS BY SIDRATUL MUNTAHA BINTE ANAM OTITHI, Student No. 2305009, Session: 2023-2024; MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY, DEPARTMENT OF PLANT PATHOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR; JUNE 2024. 2024-06-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2086 EFFECT OF FERTILIZER AND PLANT GROWTH REGULATOR ON LEAF BLIGHT OF WHEAT Jahan, Kawser A field experiment was conducted at the research field of Agricultural Research Station (BARI), Dinajpur during period of December 2023 to April 2024 to evaluate the effect of fertilizer and PGR application on leaf blight disease of wheat. Randomized complete block design with four replications and five treatments was followed for the experiment. The variety of BARI Gom 33 was used in this experiment. The treatments used in the experiment were T1:100% FRG recommended dose (control), T2: FRG recommended dose (100% urea+85% TSP +100% MoP) + ROOTOPEX, T3: FRG recommended dose (100% urea+75% TSP +100% MoP) + ROOTOPEX, T4: FRG recommended dose (100% urea+50% TSP +100% MoP) +ROOTOPEX and T5: Farmers practice (650g urea+550g TSP+450g MoP). In this experiment after 95 DAS, percentage of disease leaf area and severity both were found the highest at T1. In case of percentage of normal seeds, T2 showed the highest result having 73.75% where Rootopex was applied. Percentage of undersized seeds were recorded the highest at T1 having 20.50%. On the other hand, percentage of broken and black pointed seeds were found the highest at T5. In case of total number of grains per spike, T2 showed the highest percentage. About yield, the highest amount noted at T2 having 12.32kg/plot . In case of seed health seeds, seeds from Rootopex applied plot showed very impressive result like the highest percentage of germination recorded at T2 having 92%. When it comes to percentage of pathogen associated with seeds, T5 showed maximum valued. In this experiment fertilizer combined with plant growth regulator (PGR) showed positive response among maximum parameters. EFFECT OF FERTILIZER AND PLANT GROWTH REGULATOR ON LEAF BLIGHT OF WHEAT; A THESIS BY Kawser Jahan, Student No.: 2305008, Session : 2023-2024, Thesis Semester: January – June 2024, MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY, DEPARTMENT OF PLANT PATHOLOGY, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; JUNE 2024. 2024-06-01T00:00:00Z