http://103.7.193.12:8080/xmlui/handle/123456789/137 2026-07-26T09:56:07Z http://103.7.193.12:8080/xmlui/handle/123456789/2159 DEVELOPMENT AND EVALUATION OF ADVANCED GENERATIONS TO EVOLVE HIGH YIELDING VARIETIES OF JUTE (Corchorus olitorius L.) Biswas, Sanjoy Kumar Several experiments were conducted on Tossa jute (Corchorus olitorius L) at Jute Agricultural Experimental Station, Jagir, Manikgonj during 2015-2018. A total of 53 diverged genotypes were included to study on 11 characters like plant height (cm), base diameter (mm), green bark thickness (mm), leaf length (cm), leaf width ( mm), leaf angle (0), petiole length (cm), green weight with leaves (g), green weight without leaves (g), fibre weight (g) and stick weight (g) to create segregants from where outstanding advanced lines may evolve for the development new varieties. Significant variations were observed for all characters among the genotypes. Simultaneous consideration of high mean performance and high heritability showed by green bark thickness, green weight without leaves, plant height and base diameter are necessary to the breeders for improving Tossa jute through selection breeding. The fifty three genotypes were grouped into five clusters by D2 statistics which were confirmed through superimposing corresponding PCAs. The cluster III accommodated the highest number of genotypes (18) and cluster I included only three genotypes. Both upper and lower magnitudes of expression for characters like base diameter, plant height, green bark thickness and late flowering were considered to produce diversified segregants. Accordingly 8 parents, O-9897, O-795, JRO-524, Acc-2381, Acc-3423, Acc.-3438 Acc-3533 and Acc-3860 were selected simultaneous consideration of clustering and phenotypic acceptance for full diallel crossing program and 8 x 7=56 experimental hybrids were produced. On the way of evaluation, mean performances, correlation coefficients and path analysis of each character on fibre yield were estimated to assess the nature relation pertained among the characters. In general, genotypic correlation coefficients were higher than concurrent phenotypic correlation coefficients, indicated preponderance effects genetic makeup upon expression of the characters. Out of 8 parents, 4 parents Acc-2381, Acc 3423, Acc-3533 and Acc-3860 produced higher fibre weight but positive and significant GCAs were estimated for O-795, JRO-524 Acc-3533 and Acc-3860. Besides, consideration means and GCAs, two lines Acc-3533 and Acc-3860 also appeared as good combiners in both directions hybridization program. Reciprocal crosses apparently exhibited significant cytoplasmic gene effects to express the selected characters during field evaluation. Seven hybrid combinations from normal and another seven combinations from reciprocal crosses revealed significant and positive SCAs effects for fibre weight/plant. The positive and significant SCAs were resulted due to additive and non-additive interactions of the concerned alleles. The joint consideration of positive and significant GCAs and SCAs the crosses P2 x P6 (O-795 x Acc-3438), P2 x P7 (O-975 x Acc-3533), P3 x P4 (JRO-524 x Acc-2381), P3 x P5 (JRO-524 x Acc-3423), P7 x P8 (Acc-3533 x Acc-3860), P5 x P2 (Acc-3423 x O-975) , P6 x P3 (Acc-3438 x JRO-524), P7 x P5 ( Acc-3533 x Acc-3423, P8 x P2 (Acc-3860 x O-975) and P8 x P4 (Acc-3060 x Acc-2381) were outstanding for improving fibre yield potential in Tossa jute through cross breeding programs. From each of the F2 generation against a cross a total of 300 plants were initially selected and then variability parameters based 10% plants (30 plants) were selected to advance in next generation. The segregating populations derived from P8 x P4 (Acc-3060 x Acc-2381) and P3 x P4 (JRO-524 x Acc-2381) might produce superior segregants in advanced generation, therefore, the breeders may exploit the segregants to operate either pedigree or bulk method to evolve outstanding advanced lines of Tossa jute. DEVELOPMENT AND EVALUATION OF ADVANCED GENERATIONS TO EVOLVE HIGH YIELDING VARIETIES OF JUTE (Corchorus olitorius L.); A PhD DISSERTATION By Sanjoy Kumar Biswas, Registration No. 1505306, DEPARTMENT OF GENETICS AND PLANT BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR-5200; NOVEMBER, 2021. 2021-11-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2156 STUDY ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCES AND SEMEN QUALITY OF ASEEL CHICKEN PRAMANIK, Md. AHSAN HABIB Aseel, Naked Neck, and Red Junglefowl are the most important chicken genetic resources in Bangladesh. However, due to the unconsciousness of the farmers, uncontrolled crossing with other indigenous chickens, lack of proper conservation programs these are close to extinction. To conserve the extinct species the various ways were adopted in developed countries. As the first initiative of in vitro conservation of the poultry genetic resources, the present study was undertaken with four different distinct experiments to know the productive and reproductive performance of Aseel chicken and to study semen quality, sperm motility, biokinetic characteristics, sperm cryo-survivability by pre-freezing addition of honey as the possible cryo-protectant. Semen quality and the effect of honey on semen cryopreservation were evaluated by measuring sperm motility and biokinetic parameters with Computer Semen Analysis (CASA). The Aseel, Naked Neck and Red Junglefowl chickens were reared in open sided house in semi-scavenging condition at the Advanced Avian Research Farm, HSTU, Dinajpur. The Aseel sperm were cryopreserved using different level of honey where Glycerol and DMSO were also used as the positive controls. Considering productive and reproductive characteristics of the Aseel chicken showed almost similar performances of other chicken genetic resources but in some cases Aseel performed better. The semen color obtained from Aseel cocks was creamy white and showed a normal appearance and proved the semen quality was normal. The volume of semen per ejaculation and the pH were almost similar to others’ semen. Sperm motility was also observed normal in relation to motility and progressive motility along with other biokinetic characteristics. Considering the motility and percentage of the progressively motile sperm, the semen samples might be stored for up to 2 hours in good condition for insemination. Pre-freezing addition of 3% honey showed the satisfactory results in maintaining cryo-survivability. The productive and reproductive characteristics did not vary in case of natural and artificial insemination with fresh and diluted semen. Moreover, the result showed that the addition of 3% honey in Ringer’s solution performed as a cryoprotective agent which may help to cryopreserve Aseel roosters’ semen as the native chicken genetic resource and revealed that honey might has the cryoprotective properties. Therefore, the study concluded that the pre-freezing addition of honey improved the cryo-survivability of Aseel’s sperm and suggested for in vitro conservation of chicken genetic resources available in Bangladesh. STUDY ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCES AND SEMEN QUALITY OF ASEEL CHICKEN; Dissertation submitted By Md. AHSAN HABIB PRAMANIK, Registration No.: 1805486; Doctor of Philosophy (PhD) in Genetics and Animal Breeding. Department of Genetics and Animal Breeding. Hajee Mohammad Danesh Science and Technology University Dinajpur-5200, Bangladesh; January 2023. 2023-01-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2150 MULTIVARIATE ANALYSIS AND EVALUATION OF HYBRIDS WITH THEIR PARENTS IN FINE RICE Haque, Md. Monzurul Twenty five fine rice genotypes including thirteen non-aromatic and twelve aromatic cultivars were evaluated for fifteen quantitative characters to select the most yield enhancing characters as well as the diverged cultivars for hybridization. The mean performances and genetic parameters of the characters were separated through univariate analysis; the most yield promoting characters were selected by bivariate and multivariate analyses and desirable parents were selected by univariate and multivariate analyses. Though high heritability was estimated for most the characters but the highest genetic advance was estimated against spikelets/panicle (125.74). Effective tillers/hill, spikelets/panicle showed positive and significant, and plant height, days to 50% flowering and days to maturity showed negative and significant correlation coefficients with yield. Upon partitioning the total phenotypic correlation coefficients with yield, 1000-grain weight exerted the highest direct effect (0.84), followed by spikelets/panicle (0.67) and effective tillers/hill (0.61), therefore, these three characters appeared as the predominant yield accelerating characters in fine rice. A total of thirty selection indices were constructed based on five characters including yield and the highest relative efficiency of simultaneous selection was gained from I12345 (223.31%). Twenty five cultivars were grouped into six clusters by D2 statistics and six cultivars such as Kataribhog, Salna, Ranjit, Begunbichi, Chinigura and Kalozira were selected based on clustering as well as rank distribution of the cultivars. The selected parental lines were evaluated both in research and farmer’s fields to assess their tangible performance for the selected characters. The estimated yield gap between the research and the farmer’s fields was 0.86 t/ha. The selected cultivars produced significantly higher effective tillers/hill, spikelets/panicle and yield in the research field than that of farmer’s field. However, the potentiality of the segregants was predicted in the course of such comparison. It was initially aimed to develop fifteen experimental hybrids through a diallel model, but eight hybrids were finally produced from these six parental lines. The cytogenetical barrier against the failure of seven hybrids was investigated. The non remarkable karyotypic disharmony in the parents may not be the principal cause behind the failure of seven hybrids; other reasons like genetical, physiological and environmental factors may be responsible to create such partial hybridization failure that need to be further investigation. The eight hybrids were evaluated and their competence was measured by three criteria of heterosis. The aroma contents in the hybrids and their parents were compared. Out of eight hybrids, three were non-aromatic and five were aromatic, in which the highest aroma was assessed in the F1 of Kataribhog × Salna and F1 of Kataribhog × Chinigura, hence consideration of yield and aroma content these two hybrids may bring to obtain desirable segregants. MULTIVARIATE ANALYSIS AND EVALUATION OF HYBRIDS WITH THEIR PARENTS IN FINE RICE; A PhD DISSERTATION by Md. Monzurul Haque, Registration no. : 1005053; DEPARTMENT OF GENETICS AND PLANT BREEDING, FACULTY OF AGRICULTURE, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; FEBRUARY, 2014. 2014-02-01T00:00:00Z http://103.7.193.12:8080/xmlui/handle/123456789/2148 GENETIC ANALYSIS OF YIELD AND YIELD CONTRIBUTING TRAITS IN PUMPKIN (Cucurbita moschata Duch. Ex. Poir) GAZI, MD. MOHSIN The present research program was undertaken to study the genetic analysis of yield and yield contributing traits of pumpkin (Cucurbita moschata Duch. ex Poir.). All experiments were conducted at R & D Farm, Lal Teer Seed Limited, Gazipur, Bangladesh. The present study includes genetic diversity study, interrelationships among yield components, combining ability, heterosis, and heritability of yield and yield related traits. Direct and indirect effects of the traits to yield were also analyzed. Detailed gene actions were studied in selected four crosses for yield and yield contributing characters. The tested genotypes exhibited wide range of variation on different yield and yield components. Among twenty one genotypes of pumpkin, six performed better in respect of different yield components and quality characters. For all traits, genotypic coefficient of variation (GCV) was always smaller than phenotypic coefficient of variation (PCV). Narrow differences between GCV and PCV and high heritability coupled with high genetic advance (GA) confirmed least environmental effects on fruit length, single fruit weight, brix content and yield per plant which offered better scope of selection for variety improvement program of pumpkin. Based on the degree of divergence among genotypes, the twenty one genotypes were grouped into six clusters. Out of these, six different divergent inbred parents (CM-20, CM-1, CM-5, BARI Mistikumra-1, CM-8 and CM-14) were selected using ranking on variance among genotypes within cluster for 6X6 diallel cross including reciprocals. Yield per plant, at phenotypic and genotypic level, was significantly and positively correlated with fruit length, fruit diameter, single fruit weight, flesh thickness, number of fruits per plant and 100-seed weight. These components had the maximum contribution towards total divergence. The direct effect of single fruit weight was very close to correlation value at phenotypic and genotypic level. Thus selection was done through single fruit weight and number of fruits per plant. Studies on interrelationships among yield and yield components revealed that genetic correlation coefficient was higher than corresponding phenotypic ones for all the characters studied, thereby establishing strong inherent relationships among them. Path coefficient analysis showed that single fruit weight had maximum direct effect on yield per plant followed by number of fruits per plant, days to female flower per plant, fruit diameter, number of seeds per fruit and number of female flowers per plant both at genotypic and phenotypic levels. Significant GCA variance was recorded for days to male and female flower opening, fruit length and diameter, single fruit weight, number of fruits per plant, number of seeds per fruit, 100 seed weight, brix content and yield. Both additive and non-additive effects influenced the performance of the hybrid for all traits. Four parents (CM-1, BARI Mistikumra-1, CM-20 and CM-8) were found as good general combiners. Obtaining significantly higher and positive heterosis and expected negative effects for different important characters of pumpkin, two cross combinations namely, CM-1 X BARI Mistikumra-1 and CM-8 X CM-1 were identified as the promising hybrids commercial utilization. Thus, these two hybrids were approved and registered as Hajee F1 and Danesh F1 respectively by NSB, MOA of Bangladesh. Indigenous X Exotic crosses showed significant greater heterosis for yield and its components. Broad sense heritability of all the 13 characters was above 90% indicating high heritability since the expression of yield and its components were controlled by additive, dominance and different epistatic gene interactions which can be used in desirable direction for varietal improvement program of pumpkin. GENETIC ANALYSIS OF YIELD AND YIELD CONTRIBUTING TRAITS IN PUMPKIN (Cucurbita moschata Duch. Ex. Poir); A DISSERTATION BY GAZI MD. MOHSIN, REGISTRATION NO. 1105044; DOCTOR OF PHILOSOPHY IN GENETICS AND PLANT BREEDING, DEPARTMENT OF GENETICS AND PLANT BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR, BANGLADESH; APRIL, 2015 . 2015-04-01T00:00:00Z