DEPARTMENT OF MICROBIOLOGY (MIC) http://103.7.193.12:8080/xmlui/handle/123456789/47 2026-04-17T16:56:40Z 2026-04-17T16:56:40Z Hossain, Md. Khaled http://103.7.193.12:8080/xmlui/handle/123456789/1880 2022-05-18T08:06:13Z 2014-01-01T00:00:00Z

Circulating Tumor Cell Chip in Breast Cancer for Theragnosis based on Surface—Enhanced Raman Spectroscopy Hossain, Md. Khaled Circulating Tumor Cell Chip in Breast Cancer for Theragnosis based on Surface-Enhanced Raman Spectroscopy Dissertation Director: Professor Jeong-Woo Choi Circulating tumor cells (CTCs) are emerging biomarkers, especially in case of liquid biopsy, and important indicator for prognosis monitoring in case of personalized anticancer therapy. CTC analysis is a promising diagnostic method for estimating the risk of metastatic relapse and metastatic progression in patient with cancer. The basic problem of CTC study is their extremely low inherent numbers in blood (around one CTC per 10° non cancerous hematopoietic cells). Hence, before detection or characterization of CTCs their isolation is important. A subpopulation of CTCs with stemlike behavior are known as stem-like circulating tumor cells (SCTCs). In recent years stem like cancer cells (SCCs) hypothesis has attracted great attention in the field of cancer biology. According to the concept, a minor Circulating Tumor Cell Chip in Breast Cancer for Theragnosis based on Surface—Enhanced Raman Spectroscopy Md. Khaled Hossain

2014-01-01T00:00:00Z SALAD, SAID MOHAMED http://103.7.193.12:8080/xmlui/handle/123456789/1727 2022-05-17T04:43:28Z 2019-12-01T00:00:00Z

ISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICTISOLATION AND IDENTIFICATION OF BACTERIAL PATHOGENS FROM RAW CHICKEN MEAT IN DINAJPUR DISTRICT SALAD, SAID MOHAMED The present research work was under taken to isolate and identify the associated bacteria of chicken raw meat and to find out the effective antibiotics against the bacteria through antibiogram studies in Dinajpur district Bangladesh. July to November, 2019. A total of 48 samples were randomly collected from markets and transported to the Microbiology laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur. After processing of samples primary culture was done in nutrient broth and nutrient agar then pure culture was obtained from different selective media. The level of bacterial load were significantly higher the first sampling in comparison with the second and third one respectively. The prevalence of Escherichia coli, Salmonella spp. and Staphylococcus spp. were 47.9%, 39.6% and 12.5% respectively. All pure isolates were subjected to Antibiogram assay test by disc diffusion method against 8 different antibiotics. E. coli isolates were sensitive moxifloxacin. Highest resistant to chloraphenicol, tetracycline and streptomycin. Among all Salmonella spp., isolates, chloraphenicol showed the highest susceptibility pattern followed by the tetracycline, azithromycin, ampicillin and Erythromycin. Highest resistant pattern was showed by penicillin, Cefalexin and amoxicillin. In case of Staphylococcus spp., chloraphenicol showed the highest susceptibility pattern followed by the streptomycin and streptomycin found sensitive in this study. Highest resistant pattern showed by the tetracycline, azithromycin, ampicillin and tetracycline. This study revealed that broiler meat sold at some local markets in Dinajpur city were contaminated with multiple species of multidrug resistant bacteria which may risk for human health. A THESIS BY SAID MOHAMED SALAD REGISTRATION NO. 1805501 SEMESTER: JULY-DECEMBER, 2019 SESSION: 2018 Submitted to the Department of Microbiology Hajee Mohammad Danesh Science and Technology University, Dinajpur In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (M.S.) IN VETERINARY PUBLIC HEALTH AND FOOD HYGIENE

2019-12-01T00:00:00Z ROY, MITHUN CHANDRA http://103.7.193.12:8080/xmlui/handle/123456789/1714 2022-05-17T03:49:47Z 2019-06-01T00:00:00Z

DETEMINATION OF MICROBIAL QUALITY OF DRINKING WATER OBTAINED FROM DIFFERENT SOURCES FOR HUMAN, POULTRY AND DAIRY FARM IN DINAJPUR DISTRICT OF BANGLADESH ROY, MITHUN CHANDRA Total ninety (90) samples, thirty 30 from human tubewell water, 30 water samples of dairy farm and 30 water samples of poultry farm were collected and tested for TVC and MPN to to determine the microbial quality of drinking water. The mean HPC of human tubewell water were found 2.55×103 , 3.11×103 , 2.85×103 , 2.88×103 and 3.20×103 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest HPC was found in Chirirbandar 3.20×103 CFU mL-1 and lowest found in Sadar 2.55×103 CFU mL-1. The MPN values were found 2.16 , 2.16 , 2.50 , 1.83 and 2.33 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest MPN value was found in Kaharol 2.50 and lowest were found in sadar 2.16 and Chirirbandar 2.16 coliforms/100 ml .The mean HPC of dairy farm tubewell water were found 2.3×103 , 3.6×103 , 2.7×103 , 3.2×103 and 3.4×103 CFU mL-1 in Sadar, Birgonj, Kaharol ,Birol and Chirirbandar .The highest HPC of dairy farm tubewell water was found in Birgonj 3.6×103 CFU mL-1 and lowest HPC was found in Sadar 2.3×103 CFU mL-1. The MPN values were found 1.66, 2.33, 2.66, 2.00 and 2.66 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The highest MPN values were found in Kaharol 2.66 coliforms/100 ml and Chirirbandar 2.66 coliforms /100 ml and lowest was found in sadar 1.66 coliforms/100 ml of water.The mean of HPC of dairy farm manger water were in 4.66×107 , 2.0×107 , l 2.8×107 , 2.8×107 and 4.2×107 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively.The highest mean HPC of dairy farm manger water was found in Sadar 4.66×107 CFU mL-1 and lowest found in Birgonj 2.0×107 CFU mL-1 .The MPN values were found 14.00, 17.33, 14.00, 16.33 and 15.33 coliforms /100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The MPN of dairy farm manger water was highest in Birgonj 17.33 coliforms /100 ml and lowest were found in Sadar 14.00 coliforms /100 ml and Kaharol 14.00 coliforms/100 ml.The geometric mean of HPC of Poultry farm (Tubewel/Machine) water were Sadar 2.8×103 CFU mL-1 , Birgonj 2.67×103 CFU mL-1 , Kaharol 3.0×103 CFU mL-1 , Birol 2.57×103 CFU mL-1 and Chirirbandar 3.0×103 CFU mL-1 .In this study, it was found that HPC of Poultry farm tubewell water was highest in Kaharol 3.0×103 CFU mL-1 and lowest in Birol 2.57×103 CFU mL-1 .The MPN values of Poultry farm tubewell water were found 2.66, 2.00, 2.33, 1.66 and 2.00 coliforms/100 ml of drinking water in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The MPN of Poultry farm tubewell water was highest in Sadar 2.66 coliforms/100 ml and lowest in Birol 1.66 coliforms/100 ml.The mean of HPC of Poultry farm waterer water were found 4.2×107 , 2.00×107 , 3.25×107 , 1.00×107 and 2.00×107 CFU mL-1 in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. Highest HPC of Poultry farm waterer water was found in Sadar 4.2×107 CFU mL-1 and lowest in Birol 1.00×107 CFU mL-1 . The MPN values of poultry waterer water were 12.33, 9.66, 10.33, 13.33 and 12.33 coliforms/100 ml in Sadar, Birgonj, Kaharol, Birol and Chirirbandar respectively. The highest MPN value was found in Birol 13.33 coliforms/100 ml and lowest were found in Birol 9.66 coliforms/100 ml. From ninety water samples seven bacteria were isolated among them the most frequent isolate was Escherichia coli 82(15.95%) followed by Salmonella spp 81(15.75%), Shigella spp 80(15.56%), Klebsiella spp 79(15.36%), Vibrio spp 73(14.20%), Pseudomonas spp 60(11.67%) and Staphylococcus spp 59(11.47%). The antimicrobial susceptibility pattern showed that the isolates were highly resistant to ampicillin, amoxicillin, erythromycin and chloramphenicol and susceptible to gentamicin, azithromycin, colistin, ceftriaxone, levofloxacin and ciprofloxacin. Out of seven bacteria two bacteria (Escherichia coli and Vibrio spp) are subjected to amplified by using 16S rRNA gene based PCR A THESIS BY MITHUN CHANDRA ROY REGISTRATION NO. 1705432 SEMESTER: JANUARY-JUNE, 2019 SESSION: 2017 Submitted to the Department of Microbiology Hajee Mohammad Danesh Science and Technology University, Dinajpur In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (M.S.) IN Veterinary Public Health and Food Hygiene

2019-06-01T00:00:00Z SALMUN RIFAT, SYEDA http://103.7.193.12:8080/xmlui/handle/123456789/1670 2022-05-16T08:18:56Z 2015-05-01T00:00:00Z

BACTERIAL LOAD IN FECAL SAMPLE AND GROWTH PERFORMANCE OF SHEEP AT GOBINDAGONJ UPAZILLA OF GAIBANDHA DISTRICT OF BANGLADESH SALMUN RIFAT, SYEDA The present study was conducted to identify the organisms in feces of sheep and their effects on growth performance of sheep at Gobindogonj Upazilla, Gaibandha. The samples were analyzed to determine the total viable count, prevalence of gram negative bacteria, Eschrechia coli and Salmonella spp. The sheep were categorized into three (3) groups namely group A, group B and group C according to their body weight gain at same age. For the determination of bacterial load a total of 45 samples were tested where E.coli present in 20 sample and prevalence was 44.44%, ( group A- 60%, Group B -40% and group- C 33.33%), and Salmonella spp was present in 11 samples and prevalence was 24.44% (group A- 33.33%, Group B- 26.67% and group C-13.33%). From this study the result represented that the total viable counts/g feces in group A, group B and group C were 9.8 x 106, 4.9x 104 and 3.4x 103 CFU/g sample respectively, the E. coli counts/g feces in group A, group B and group C were 4.24 x 106, 2.4x 104 and 5.5x 102 CFU/g of sample respectively and Salmonella spp. counts/g sample on group A, group B and group C were 3.2 x 103, 2.1x 102 and 1x 102 CFU/g feces respectively. The study showed that group A had highest bacterial load (6.99 Log 10/g) and group C had lowest bacterial load (3.53Log10/g).In conclusion it is evident that E. coli and Salmonella spp was successfully detected through different bacteriological agar viii media and biochemical reaction indicating the isolated organisms were responsible for possible faecal pollution and also a negative effect on growth performance of sheep in reared in research area. A THESIS BY SYEDA SALMUN RIFAT Registration No.: 1305067 Semester: January-June/2015 Submitted to the Department of Microbiology Faculty of Post Graduate Studies Hajee Mohammad Danesh Science and Technology University, Dinajpur, In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (M.S.) IN MICROBIOLOGY

2015-05-01T00:00:00Z