DEPARTMENT OF GENETICS & PLANT BREEDING (GPB) http://103.7.193.12:8080/xmlui/handle/123456789/15 2026-07-26T08:19:35Z 2026-07-26T08:19:35Z HASAN-UZ-ZAMAN http://103.7.193.12:8080/xmlui/handle/123456789/2171 2026-06-25T06:18:20Z 2023-12-01T00:00:00Z

HUMAN DOSES OF DEHP DURING PREGNANCY INFLUENCE POST-NATAL GROWTH AND REPRODUCTION IN OFF-SPRING FEMALE MICE HASAN-UZ-ZAMAN Di (2-ethylhexyl) phthalate (DEHP) is an endocrine-disrupting chemical that interferes with reproductive function and hormone regulation. Among different exposure routes (oral ingestion, inhalation, and dermal contact), the most common routes of exposure are via oral ingestion from food packaging and the use of cosmetic products, but high levels of phthalates are also present in household dust. Thus, exposure to phthalates is ubiquitous in human populations. For the general population, it was estimated that the average total daily individual ambient exposure to DEHP ranges from 0.21 to 2.1 mg/day. Thus, the estimated range of daily human exposure to DEHP is 3–30 μg/kg/day based on urinary metabolite concentrations; however, measurements of DEHP in household dust can reach up to 700 mg/kg, potentially increasing exposure levels in certain individuals. The effect of phthalate on male reproduction is now well studied; however, the effect of phthalate on female reproduction warrants extensive investigation. Because women are exposed to phthalates at a higher level than men through their widespread use of personal care and cosmetic products, In addition, it is hypothesized that phthalate can cross the placenta and interfere with the embryo, postnatal growth and development, and the later life of offspring after parturition. In this study, the Swiss albino female mice were randomly divided into four groups namely T0 (control), T1 (2mg/kg DEHP), T2 (4mg/kg DEHP), and T3 (6 mg/kg DEHP). Mating‟s strategy was followed by two virgin females and one stud male, who were confirmed pregnant by observing a vaginal plug. DEHP was exposed from the plug confirmed to parturition. After parturition, litter size (number of pups), litter weight, weight/pups, and body weight took until sexual maturity, then evaluated the reproductive cyclicity, the hormonal profile, hematological parameters, and organ developments after sexual maturity of female mice. The result showed that from birth to weaning, offspring of the T1 group exhibited a significantly body weight gain compare to the T0 group. On the other hand, considering weaning to sexual maturity, the rate of body weight gain was higher in the T1 and T2 groups compared to the T0 group. The final body weight was higher in the T2 group compared to the offspring of the T0 group. The estrous cyclicity data revealed that the rate of estrous in the T1, T2, and T3 groups were gradually decreased compared to the T0 group, in case of proestrus T1 was increased compared to T0 group but T2, T3 group were decreased compared to T0 group. The rate of diestrus in the T1, T2 and T3 groups were increased compare to the T0 group. On the other hand the rate of metestrus in the T1 and T2 group were decreased compare to T0 group but T3 was increased compared to T0 group. In the hormonal assay, the percentage of estrogen in the T1, T2 and T3 were gradually decreased compared to T0 group and the percentages of progesterone levels in the treatment groups were decreased significantly. Hematological parameters showed that the platelets in the T1, T2, and T3 groups were significantly higher than T0 group. In the term of neutrophil percentage, the T3 group was significantly lower than the other groups, Lymphocyte percentage in the T1, T2, and T3 groups were significantly higher than the T0 group. In case of total WBC count in the T1, T2 and T3 groups were increased compared to T0 group. The hematological parameters of PCT, RDW-CV, and RDW-SD were significantly lower in the T1, T2, and T3 groups than the T0 group. The percentage of monocytes in the T1, T2 and T3 were gradually decreased compared to T0 group. In cases of organ development, lung weight in the T1 and T3 groups were significantly increased from the T0 group but the T2 group was decreased from T0 group. In the T1, T2 and T3 groups, kidney and liver weight were significantly higher than in the T0 group. Heart weight in the T2 and T3 groups were significantly higher than in the T0 group. In the same way, spleen weight is comparatively higher in the T1 and T2 groups compared to the T0 group. There were no significant changes in the thyroid, uterus, or ovaries. Visual observation of the morphological characteristics of different organs showed that slight discoloration of the liver and lung was substantially affected in T3 group compared to T0 group. HUMAN DOSES OF DEHP DURING PREGNANCY INFLUENCE POST-NATAL GROWTH AND REPRODUCTION IN OFF-SPRING FEMALE MICE; A THESIS BY HASAN-UZ-ZAMAN, Student No. 1504096, Semester: July-December, 2023, Session: 2022-2023; MASTER OF SCIENCE (M.S.) IN GENETICS AND ANIMAL BREEDING, DEPARTMENT OF GENETICS AND ANIMAL BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR-5200; DECEMBER 2023.

2023-12-01T00:00:00Z Biswas, Sanjoy Kumar http://103.7.193.12:8080/xmlui/handle/123456789/2159 2026-06-16T07:49:17Z 2021-11-01T00:00:00Z

DEVELOPMENT AND EVALUATION OF ADVANCED GENERATIONS TO EVOLVE HIGH YIELDING VARIETIES OF JUTE (Corchorus olitorius L.) Biswas, Sanjoy Kumar Several experiments were conducted on Tossa jute (Corchorus olitorius L) at Jute Agricultural Experimental Station, Jagir, Manikgonj during 2015-2018. A total of 53 diverged genotypes were included to study on 11 characters like plant height (cm), base diameter (mm), green bark thickness (mm), leaf length (cm), leaf width ( mm), leaf angle (0), petiole length (cm), green weight with leaves (g), green weight without leaves (g), fibre weight (g) and stick weight (g) to create segregants from where outstanding advanced lines may evolve for the development new varieties. Significant variations were observed for all characters among the genotypes. Simultaneous consideration of high mean performance and high heritability showed by green bark thickness, green weight without leaves, plant height and base diameter are necessary to the breeders for improving Tossa jute through selection breeding. The fifty three genotypes were grouped into five clusters by D2 statistics which were confirmed through superimposing corresponding PCAs. The cluster III accommodated the highest number of genotypes (18) and cluster I included only three genotypes. Both upper and lower magnitudes of expression for characters like base diameter, plant height, green bark thickness and late flowering were considered to produce diversified segregants. Accordingly 8 parents, O-9897, O-795, JRO-524, Acc-2381, Acc-3423, Acc.-3438 Acc-3533 and Acc-3860 were selected simultaneous consideration of clustering and phenotypic acceptance for full diallel crossing program and 8 x 7=56 experimental hybrids were produced. On the way of evaluation, mean performances, correlation coefficients and path analysis of each character on fibre yield were estimated to assess the nature relation pertained among the characters. In general, genotypic correlation coefficients were higher than concurrent phenotypic correlation coefficients, indicated preponderance effects genetic makeup upon expression of the characters. Out of 8 parents, 4 parents Acc-2381, Acc 3423, Acc-3533 and Acc-3860 produced higher fibre weight but positive and significant GCAs were estimated for O-795, JRO-524 Acc-3533 and Acc-3860. Besides, consideration means and GCAs, two lines Acc-3533 and Acc-3860 also appeared as good combiners in both directions hybridization program. Reciprocal crosses apparently exhibited significant cytoplasmic gene effects to express the selected characters during field evaluation. Seven hybrid combinations from normal and another seven combinations from reciprocal crosses revealed significant and positive SCAs effects for fibre weight/plant. The positive and significant SCAs were resulted due to additive and non-additive interactions of the concerned alleles. The joint consideration of positive and significant GCAs and SCAs the crosses P2 x P6 (O-795 x Acc-3438), P2 x P7 (O-975 x Acc-3533), P3 x P4 (JRO-524 x Acc-2381), P3 x P5 (JRO-524 x Acc-3423), P7 x P8 (Acc-3533 x Acc-3860), P5 x P2 (Acc-3423 x O-975) , P6 x P3 (Acc-3438 x JRO-524), P7 x P5 ( Acc-3533 x Acc-3423, P8 x P2 (Acc-3860 x O-975) and P8 x P4 (Acc-3060 x Acc-2381) were outstanding for improving fibre yield potential in Tossa jute through cross breeding programs. From each of the F2 generation against a cross a total of 300 plants were initially selected and then variability parameters based 10% plants (30 plants) were selected to advance in next generation. The segregating populations derived from P8 x P4 (Acc-3060 x Acc-2381) and P3 x P4 (JRO-524 x Acc-2381) might produce superior segregants in advanced generation, therefore, the breeders may exploit the segregants to operate either pedigree or bulk method to evolve outstanding advanced lines of Tossa jute. DEVELOPMENT AND EVALUATION OF ADVANCED GENERATIONS TO EVOLVE HIGH YIELDING VARIETIES OF JUTE (Corchorus olitorius L.); A PhD DISSERTATION By Sanjoy Kumar Biswas, Registration No. 1505306, DEPARTMENT OF GENETICS AND PLANT BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR-5200; NOVEMBER, 2021.

2021-11-01T00:00:00Z PRAMANIK, Md. AHSAN HABIB http://103.7.193.12:8080/xmlui/handle/123456789/2156 2026-06-14T08:46:13Z 2023-01-01T00:00:00Z

STUDY ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCES AND SEMEN QUALITY OF ASEEL CHICKEN PRAMANIK, Md. AHSAN HABIB Aseel, Naked Neck, and Red Junglefowl are the most important chicken genetic resources in Bangladesh. However, due to the unconsciousness of the farmers, uncontrolled crossing with other indigenous chickens, lack of proper conservation programs these are close to extinction. To conserve the extinct species the various ways were adopted in developed countries. As the first initiative of in vitro conservation of the poultry genetic resources, the present study was undertaken with four different distinct experiments to know the productive and reproductive performance of Aseel chicken and to study semen quality, sperm motility, biokinetic characteristics, sperm cryo-survivability by pre-freezing addition of honey as the possible cryo-protectant. Semen quality and the effect of honey on semen cryopreservation were evaluated by measuring sperm motility and biokinetic parameters with Computer Semen Analysis (CASA). The Aseel, Naked Neck and Red Junglefowl chickens were reared in open sided house in semi-scavenging condition at the Advanced Avian Research Farm, HSTU, Dinajpur. The Aseel sperm were cryopreserved using different level of honey where Glycerol and DMSO were also used as the positive controls. Considering productive and reproductive characteristics of the Aseel chicken showed almost similar performances of other chicken genetic resources but in some cases Aseel performed better. The semen color obtained from Aseel cocks was creamy white and showed a normal appearance and proved the semen quality was normal. The volume of semen per ejaculation and the pH were almost similar to others’ semen. Sperm motility was also observed normal in relation to motility and progressive motility along with other biokinetic characteristics. Considering the motility and percentage of the progressively motile sperm, the semen samples might be stored for up to 2 hours in good condition for insemination. Pre-freezing addition of 3% honey showed the satisfactory results in maintaining cryo-survivability. The productive and reproductive characteristics did not vary in case of natural and artificial insemination with fresh and diluted semen. Moreover, the result showed that the addition of 3% honey in Ringer’s solution performed as a cryoprotective agent which may help to cryopreserve Aseel roosters’ semen as the native chicken genetic resource and revealed that honey might has the cryoprotective properties. Therefore, the study concluded that the pre-freezing addition of honey improved the cryo-survivability of Aseel’s sperm and suggested for in vitro conservation of chicken genetic resources available in Bangladesh. STUDY ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCES AND SEMEN QUALITY OF ASEEL CHICKEN; Dissertation submitted By Md. AHSAN HABIB PRAMANIK, Registration No.: 1805486; Doctor of Philosophy (PhD) in Genetics and Animal Breeding. Department of Genetics and Animal Breeding. Hajee Mohammad Danesh Science and Technology University Dinajpur-5200, Bangladesh; January 2023.

2023-01-01T00:00:00Z Haque, Md. Monzurul http://103.7.193.12:8080/xmlui/handle/123456789/2150 2026-06-09T09:07:05Z 2014-02-01T00:00:00Z

MULTIVARIATE ANALYSIS AND EVALUATION OF HYBRIDS WITH THEIR PARENTS IN FINE RICE Haque, Md. Monzurul Twenty five fine rice genotypes including thirteen non-aromatic and twelve aromatic cultivars were evaluated for fifteen quantitative characters to select the most yield enhancing characters as well as the diverged cultivars for hybridization. The mean performances and genetic parameters of the characters were separated through univariate analysis; the most yield promoting characters were selected by bivariate and multivariate analyses and desirable parents were selected by univariate and multivariate analyses. Though high heritability was estimated for most the characters but the highest genetic advance was estimated against spikelets/panicle (125.74). Effective tillers/hill, spikelets/panicle showed positive and significant, and plant height, days to 50% flowering and days to maturity showed negative and significant correlation coefficients with yield. Upon partitioning the total phenotypic correlation coefficients with yield, 1000-grain weight exerted the highest direct effect (0.84), followed by spikelets/panicle (0.67) and effective tillers/hill (0.61), therefore, these three characters appeared as the predominant yield accelerating characters in fine rice. A total of thirty selection indices were constructed based on five characters including yield and the highest relative efficiency of simultaneous selection was gained from I12345 (223.31%). Twenty five cultivars were grouped into six clusters by D2 statistics and six cultivars such as Kataribhog, Salna, Ranjit, Begunbichi, Chinigura and Kalozira were selected based on clustering as well as rank distribution of the cultivars. The selected parental lines were evaluated both in research and farmer’s fields to assess their tangible performance for the selected characters. The estimated yield gap between the research and the farmer’s fields was 0.86 t/ha. The selected cultivars produced significantly higher effective tillers/hill, spikelets/panicle and yield in the research field than that of farmer’s field. However, the potentiality of the segregants was predicted in the course of such comparison. It was initially aimed to develop fifteen experimental hybrids through a diallel model, but eight hybrids were finally produced from these six parental lines. The cytogenetical barrier against the failure of seven hybrids was investigated. The non remarkable karyotypic disharmony in the parents may not be the principal cause behind the failure of seven hybrids; other reasons like genetical, physiological and environmental factors may be responsible to create such partial hybridization failure that need to be further investigation. The eight hybrids were evaluated and their competence was measured by three criteria of heterosis. The aroma contents in the hybrids and their parents were compared. Out of eight hybrids, three were non-aromatic and five were aromatic, in which the highest aroma was assessed in the F1 of Kataribhog × Salna and F1 of Kataribhog × Chinigura, hence consideration of yield and aroma content these two hybrids may bring to obtain desirable segregants. MULTIVARIATE ANALYSIS AND EVALUATION OF HYBRIDS WITH THEIR PARENTS IN FINE RICE; A PhD DISSERTATION by Md. Monzurul Haque, Registration no. : 1005053; DEPARTMENT OF GENETICS AND PLANT BREEDING, FACULTY OF AGRICULTURE, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY, DINAJPUR-5200; FEBRUARY, 2014.

2014-02-01T00:00:00Z