http://103.7.193.12:8080/xmlui/handle/123456789/112 2026-06-09T23:43:27Z 2026-06-09T23:43:27Z ZEMIN, NUSHRAT http://103.7.193.12:8080/xmlui/handle/123456789/2116 2026-05-04T05:48:16Z 2023-12-01T00:00:00Z

SELECTION OF DROUGHT TOLERANT PARENTAL LINES OF WHEAT THROUGH MORPHO-MOLECULAR DIVERSITY AND MULTI-TRAIT GENOTYPE-IDEOTYPE DISTANCE INDEX ZEMIN, NUSHRAT Drought is a major abiotic stress that significantly reduces wheat yield in the country. For this, the major aim of the study was to identify drought tolerant wheat genotypes from 100 diverse wheat genotypes. During the Rabi season of 2022–2023, the investigation was conducted at the experimental farm of Bangladesh Wheat and Maize Research Institute (BWMRI), Debiganj, Panchagarh and in the laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur and BWMRI, Dinajpur, Bangladesh. This study used a split plot design with two replications to examine twelve morpho-physiological traits: plant height, number of tillers per plant, proline content, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, root volume, root length, root shoot ratio, relative water content, and excise leaf water loss. The analysis of variances revealed significant high heritability was recorded for proline content, shoot dry weight and root dry weight. A significant positive correlation between root dry weight and shoot dry weight was observed in both control and drought conditions. Based on the morphological diversity 1HZWYT-425, 1HZWYT-430 RAJ, 11SATYN-9425, Jamal-10032, 1HZWYT-422 RAJ, BAW-1397, Jamal 9046, SABGPYT-4104, Jamal-9006 were identified in cluster I and cluster III as similar genotypes at drought condition. Jamal-9046, SABGPYT-4104, 1HZWYT-425, SABGPYT-4110, 1HZWYT 430 RAJ, 11SATYN-9425, Jamal-10032, Jamal-9006, 1HZWYT-422 RAJ and BAW-1397 were identified as drought tolerant genotypes among 100 genotypes through assessment of multi-trait genotype ideotype index (MGIDI). Based on the molecular diversity five SSR markers viz. WMS0691, GWM513, GWM495, Barc20 and Tagwm1037 were evaluated in 96 wheat cultivars for molecular characterizations. All of the SSRs were polymorphic across the 96 genotypes. In total, 24 alleles were detected and the number of alleles per locus ranged from 2 to 7 with an average of 4.8 alleles per locus. The most polymorphic alleles (seven) were produced by the marker GWM495, followed by Tagwm1037 (6), GWM513 (5), and WMS0691 (4). In contrast, Barc20 markers yielded the fewest polymorphic bands per locus of two (2). The highest PIC value (0.84) was recorded for GWM495, and GWM513 recorded second highest (0.78) and lowest by Barc20 (0.46). The genotypes 1HZWYT-425, 1HZWYT-430 RAJ, 11SATYN-9425, Jamal 10032, 1HZWYT-422 RAJ, BAW-1397, Jamal-9046, SABGPYT-4104, and Jamal-9006 were found to be comparable in cluster III, V, and VI based on molecular diversity. Results obtained from the above two study suggested that the genotypes 1HZWYT-425, 1HZWYT-430 RAJ, Jamal-9046, 1HZWYT-422 RAJ and BAW-1397 should be important genetic materials for taking any future drought stress breeding programs in wheat. SELECTION OF DROUGHT TOLERANT PARENTAL LINES OF WHEAT THROUGH MORPHO-MOLECULAR DIVERSITY AND MULTI-TRAIT GENOTYPE-IDEOTYPE DISTANCE INDEX; A THESIS BY NUSHRAT ZEMIN, STUDENT NO. 1701254, SEMESTER: July-December, 2023, SESSION: 2022-23; MASTER OF SCIENCE (MS) IN GENETICS AND PLANT BREEDING, DEPARTMENT OF GENETICS AND PLANT BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR-5200; DECEMBER 2023.

2023-12-01T00:00:00Z PAPIA, AFRIN AJAHAN http://103.7.193.12:8080/xmlui/handle/123456789/2115 2026-05-04T05:48:16Z 2023-12-01T00:00:00Z

IDENTIFICATION OF HIGH YIELDING AND STABLE BARLEY GENOTYPES THROUGH FIELD PHENOTYPING IN MULTI LOCATION TRIALS AND MOLECULAR APPROACHES PAPIA, AFRIN AJAHAN Barley (Hordeum vulgare) is a nutrient rich cereal and there is a limited number of cultivars available in Bangladesh. The study was aimed to analyze high yielding stable genotypes by genotypes environment interactions (GEI) and to locate the unique genotype by deploying molecular characterization. To get a reliable stable genotype, rather than relying on a single analysis, employing a combination of methods to measure the nature of the GEI in various dimensions is often more effective. We investigated GEI using parametric and non-parametric stability statistics including multi-trait genotype ideotype distance index (MGIDI), additive main effects and multiplicative interaction (AMMI), and GGE. Nine barley genotypes comprised of six exotic lines and three Bangladesh Agricultural Research Institute (BARI) released varieties were evaluated in a randomized complete block design across three locations (Dinajpur, Rangpur and Panchagarh) during the rabi season of 2022-2023. Various parametric and nonparametric stability statistics were calculated using a web-based STABILITYSOFT program. Based on the analysis, EEB_450, BARI Barley-7 and EEB_91 identified as superior and high yielding genotypes. The additive effects analysis of AMMI model revealed significant effects of genotype, environment, and GEI on number of grains per spike (NGPS), yield per plant (YPP) and yield per plot (YPPLOT). EEB_450 and EEB_91 for NGPS, EEB_18 and EEB_152 for YPP, and EEB_450 and EEB_91 for YPPLOT emerged as stable genotypes with optimal performance according to the AMMI model. The likelihood ratio test indicated significant effects of genotype and GEI all studied traits. Regarding NGPS, YPP and YPPLOT, EEB_450, BARI Barley-7 and BARI Barley-9 had high best linear unbiased prediction (BLUP) value and were identified as suitable genotypes. The GGE biplot method determined the most favorable location for specific genotypes. Among three experimental locations, Panchagarh was identified as the superior environment for barley cultivation with EEB_450 being the top performing genotype considering all traits. Using the multi-trait genotype ideotype distance index (MGIDI), EEB_450, BARI Barley-7 and BARI Barley-9 were deemed the most ideal genotypes. Analysis of genetic variation using SSR markers, the genotype EEB_450 revealed phenotypically stable and showed variations with the BARI released check varieties BARI Barley-7, BARI Barley-8 and BARI Barley-9. Therefore, EEB_450 is recommended as a superior performing genotype in three locations and can be released as a variety in Bangladesh. The combination of multi-location trials and molecular approaches facilitates the identification and selection of high yielding, stable barley genotypes with desirable agronomic traits. This integrated approach enhances the efficiency and precision of barley breeding programs, ultimately contributing to sustainable agricultural productivity and food security. IDENTIFICATION OF HIGH YIELDING AND STABLE BARLEY GENOTYPES THROUGH FIELD PHENOTYPING IN MULTI LOCATION TRIALS AND MOLECULAR APPROACHES; A THESIS BY AFRIN AJAHAN PAPIA, STUDENT NO. 2205097, SEMESTER: July-December, 2023, SESSION: 2022-23; MASTER OF SCIENCE (MS) IN GENETICS AND PLANT BREEDING, DEPARTMENT OF GENETICS AND PLANT BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR-5200; DECEMBER 2023.

2023-12-01T00:00:00Z MAHFUZ, ABDULLAH AL http://103.7.193.12:8080/xmlui/handle/123456789/2114 2026-05-04T05:48:16Z 2023-12-01T00:00:00Z

STUDY OF MORPHO-MOLECULAR VARIABILITY AND PATH ANALYSIS IN WHEAT (Triticum aestivum L.) MAHFUZ, ABDULLAH AL The experiment was conducted at Hajee Mohammad Danesh Science and Technology University, Dinajpur during the Rabi season 2022-2023 in RCBD design with three replications. The experimental materials were 11 wheat genotypes viz. 10 HSTU developed advance line and one check variety BARI Gom 32. Significant variations were observed in nine traits in the existing generation. The mean squares against all genotypes were significant for all the characters studied. The mean performance of all traits, the grand mean, and the range value indicated a wide range of variation among the studied genotypes for the quantitative traits. Mean values of days to 50% heading varied from 51.67 to 69.33 days. Days to 50% maturity was ranged from 102.33 to 121.33 days. The grain weight per spike (g) ranged from 1.32 to 1.88 g. The mean value of average plant height varied from 92.35 to 116.33 cm, the average spike length was 8.58 to 10.73 cm, and the average grain per spike was 30.93 to 45.20. The number of spike per 1 square meter ranged from 351.67 to 505.67 g. The minimum value of 1000 grain weight was 31.99 g and the maximum value was 47.23 g. The high-yielding line was HSTUW 1 (2812.73) whereas the low-yielding line was HSTUW 2 (2087.22). Positive significant correlation with 1000 grain weight (0.8292) at the genotypic and phenotypic levels 1000 grain weight (0.5199) but at the phenotypic level moderate positive significant association with average plant height (0.3459), and a moderate negative significant association with average grain per spike (-0.4269) to yield per plot. Path coefficient analysis indicated that the highest direct effect was observed in grain weight per spike (3.573), average spike length also exhibited a maximum positive direct effect on yield per plot (1.198) at the genotypic level but at the phenotypic level in 1000 grain weight exhibited the highest positive direct effect on yield per plot (1.108) and a strong positive significant association with yield per plot (0.520). Average grain per spike also exhibited a maximum positive direct effect on yield per plot (0.673). Average grain per spike contributed indirectly through 1000 grain weight to yield per plant. Selection through 1000 grain weight and average grain per spike may effectively increase yield per plot. Higher genotypic and phenotypic coefficient of variations were observed in grain weight per spike, number of spike, 1000 grain weight, average grain per spike, and yield per plot. High heritability was observed in days to maturity (95.85%), average plant height (93.13%), days to heading (90.78%), and moderate heritability was shown in average grain per spike (73.87%), 1000 grain weight (72.54%), number of spike per 1 square meter (72.17%), grain weight per spike (66.47%), and yield per plot (63.73%). High genetic advance as percentage of mean was observed for the number of spike per 1 square meter (21.77%), grain weight per spike (21.38%), and 1000 grain weight (20.44%). Four SSR markers were employed in 11 wheat genotypes for molecular characterization. All SSRs were polymorphic across the 11 genotypes. Thirty-one alleles were detected with an average of 7.75 alleles per marker. The most polymorphic microsatellite markers were Gwm495, Barc20, TaBarc101, and Xwmc112 each with 6 to 9 alleles. The highest Polymorphic Information Content (PIC) value 0.87 was estimated in Gwm495. In population genetic structure analysis, population I consisted of 27.27% of total genotypes i.e. 3 genotypes were pure, where not found any admixture. Population II comprised 72.73% of total genotypes i.e. 8 genotypes; 7 genotypes were found pure and 1 genotype was found heterogeneous. Cluster analysis using the UPGMA method delineated the 11 genotypes into two clusters. HSTUW 2 and HSTUW 3 are genetically more similar while HSTUW 7 and HSTUW 8, HSTUW 10, and HSTUW 1 show a minimum of showed more divergent. Finally, the significant variability present of HSTU developed existing lines, 1000 grain weight, and Average grain per spike are two very good traits, based on which will help us to select the breed later. STUDY OF MORPHO-MOLECULAR VARIABILITY AND PATH ANALYSIS IN WHEAT (Triticum aestivum L.); A THESIS BY ABDULLAH AL MAHFUZ, STUDENT NO. 2205012, SEMESTER: July-December, 2023, SESSION: 2022-23; MASTER OF SCIENCE (MS) IN GENETICS AND PLANT BREEDING, DEPARTMENT OF GENETICS AND PLANT BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR-5200; December 2023.

2023-12-01T00:00:00Z FAME, MITHILA FAHRIN http://103.7.193.12:8080/xmlui/handle/123456789/2113 2026-05-04T05:48:16Z 2023-12-01T00:00:00Z

GENETIC PARAMETERS AND CHARACTER ASSOCIATION STUDIES IN CHEMICALLY INDUCED MUTANTS OF UPLAND COTTON (Gossypium hirsutum L.) FAME, MITHILA FAHRIN The experiment was carried out at the Cotton Research, Development, Training, and Seed Multiplication Farm, Sadarpur, in 2022-2023 cropping season. The chemical mutagen, sodium azide (NaN3) was used to treat the seeds of five varieties, namely CB15. CB16, CB17, CB18 and CB19. Three concentrations viz. 10Mm/L, 15Mm/L and 25Mm/L were employed to create mutant lines. According to RCBD design, the treated seeds were sown on July, 13, 2022. Ten characters were studied to assess variations present in the mutants. Before, the first boll was picked 230 suspected mutant plants had been found. In both varieties and the mutant plants the selected characters were measured. The variety, CB18 produced the highest number of suspected mutants (51), whereas CB15 was refractory to changing its genetic makeup as produced the lowest number of mutants (37). The highest seed cotton yield was obtained from the genotype CM 13 with 232.733 g followed by CM 14, CM 9, and CB 17 and the highest lint fiber yield was recorded CM 13 with 213.2g followed by CM 14, CM 7, CM 9, and CB 17. The important characters, like the lowest plant stature (112.53 cm) and the highest sympodial branches/plant (23.133) were recorded in the mutants of CB 17. The highest bolls/plant (47.6) and the highest seeds/boll (31.866) were obtained from CM 7 and CM 11 mutants, respectively. Hence, a complicated mechanism including genetic interaction and the environment was responsible for developing early boll-picking bur short-duration cotton varieties. Last but not least, it may be suggested that early generation may not be effective, so the mutants must advance with a view to rapid fixation of heterozygous alleles which ultimately might promote the breeding exercise to develop high lint fiber yielding, short duration, and compact canopy structured cotton varieties that would eventually meet up the demand of the cotton growers in the northern belt of Bangladesh. GENETIC PARAMETERS AND CHARACTER ASSOCIATION STUDIES IN CHEMICALLY INDUCED MUTANTS OF UPLAND COTTON (Gossypium hirsutum L.); A THESIS BY MITHILA FAHRIN FAME, STUDENT NO: 2205013, SEMESTER: JULY-DECEMBER, 2023, SESSION: 2022-2023; MASTER OF SCIENCE (MS) IN GENETICS AND PLANT BREEDING, DEPARTMENT OF GENETICS AND PLANT BREEDING, HAJEE MOHAMMAD DANESH SCIENCE AND TECHNOLOGY UNIVERSITY DINAJPUR-5200; DECEMBER 2023.

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